Abstract
The volume changes of guard cell protoplasts which correlate with stomatal movements (Schnabl et al. 1978) are accompanied by a reversible starch-malate transformation. The process of starch breakdown is paralleled by a concomitant malate synthesis during the K+ -induced swelling, while the disappearance of malate is followed by starch accumulation during shrinkage (Schnabl 1980).
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References
Ben-Basset D, Anderson LE (1981) Light-induced release of bound glucose-6-phosphate dehydrogenase to the stroma in pea chloroplasts. Plant Physiol (Bethesda) 68:279–283
Charles SA, Halliwell B (1981) Light activation of fructose bisphosphatase in isolated spinach chloroplasts and deactivation by hydrogen peroxide. Planta (Berl) 151:242–246
Davies DD (1979) The central role of phosphoenolpyruvate in plant metabolism. Annu Rev Plant Physiol 30:131–158
Hampp R, Schnabl H (1984) Adenine and pyridine nucleotide status of isolated Vicia guard cell protoplasts during K+-induced swelling. Plant Cell Physiol 25(7):1233–1239
Hampp R, Goller M, Ziegler H (1982) Adenylate levels, energy charge, and phosphorylation potential during dark/light transition in chloroplasts, mitochondria and cytosol of mesophyll protoplasts from Avena sativa L. Planta (Berl) 62:448–455
Hampp R, Goller M, Füllgraf H (1984) Determination of compartmented metabolite pools by a combination of rapid fractionation of oat mesophyll protoplasts and enzymatic cycling. Plant Physiol (Bethesda) 75:1017–1021
Lowry OH, Passonneau JV (1972) A flexible system of enzymatic analysis. Academic, New York
Mansfield TA, Travis AJ, Jarvis RG (1981) Responses to light and carbon dioxide. In: Jarvis PG, Mansfield TA (eds) Stomatal physiology. Cambridge Uni Press, Cambridge, pp 119–135
O’Leary MH (1982) Phosphoenolpyruvate carboxylase: an enzymologist’s view. Annu Rev Plant Physiol:298–315
Outlaw WH, Kennedy J (1978) Enzymic and substrate basis for the anaploertic step in guard cells. Plant Physiol (Bethesda) 62:648–652
Outlaw WH, Manchester J (1979) Guard cell starch concentration quantitatively related to stomatal aperture. Plant Physiol (Bethesda) 64:79–82
Schnabl H (1980) CO2 and malate metabolism in starch-containing and starch-lacking guard cell protoplasts. Planta (Berl) 149:52–58
Schnabl H (1981) The compartmentation of carboxylating and decarboxylating enzymes in guard cell protoplasts. Planta (Berl) 152:307–313
Schnabl H, Kottmeier C (1984) Properties of phosphoenolpyruvate carboxylase in desalted extracts from isolated guard cell protoplasts. Planta (Berl) 162:220–225
Schnabl J, Bornman CH, Ziegler H (1978) Studies in isolated starch-containing and starchdeficient guard cell protoplasts. Planta (Berl) 143:33–39
Schnabl H, Elbert C, Krämer G (1982) The regulation of the starch-malate balances during volume changes of guard cell protoplasts. J Exp Bot 33:996–1003
Stitt M, ap Rees T (1979) Capacities of pea chloroplasts to catalyse the oxidative pentose phosphate pathway and glycolysis. Phytochemistry (oxf) 18:1905–1911
Smith TE (1977) Escherichia coli phosphoenolpyruvate carboxylase: studies on the mechanism of multiple allosteric interactions. Arch Biochem Biophys 183:538–552
Thorpe N, Brady CJ, Milthorpe FL (1978) Stomatal metabolism: primary carboxylation and enzyme activities. Aust J Plant Physiol 5:485–493
Willmer CM, Pallas JE, Black CC (1973) Carbon dioxide metabolism in leaf epidermal tissue. Plant Physiol (Bethesda) 52:448–452
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© 1985 Springer-Verlag Berlin Heidelberg
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Schnabl, H. (1985). Regulation of Volume Changes in Guard Cell Protoplasts. In: Pilet, PE. (eds) The Physiological Properties of Plant Protoplasts. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70144-3_19
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DOI: https://doi.org/10.1007/978-3-642-70144-3_19
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