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Effect of UV Irradiation on Different Partial Reactions of the Primary Processes of Photosynthesis

  • G. Renger
  • M. Voss
  • P. Gräber
  • A. Schulze
Part of the NATO ASI Series book series (volume 8)

Abstract

Spinach (Spinacia oleracea L. cv. Matador) chloroplasts were irradiated with several levels of UV-B and UV-C radiation. The site of impairment of the electron transport in photosystem II was localized. Electrochromic absorption changes at 520 nm, absorption changes of the primary photosystem II acceptor at 320 nm, dichlorophenolindophenyl reduction by photosystem II, oxygen yield per flash, and variable fluorescence and herbicide binding (atrazine, 3-(3,4-dichlorodiphenol)-1,1 dimethylurea [DCMU]) were measured in normal and UV-treated chloroplasts. A decrease of electron transport and variable fluorescence, and also a decreased binding constant for DCMU-type inhibitors was observed after UV treatment. It is suggested that UV irradiation primarily modifies the binding protein of the primary and secondary plastoquinones of PS II acceptor side (QA-QB apoprotein), simultaneously leading to a functional blocking of the primary acceptor. Except for the quantum efficiency, UV-B and UV-C act at this site in a similar way.

A striking difference between UV-C and UV-B action was observed in the correlation between variable fluorescence and 2,6-dichlorophenol-indophenol (DCIP) reduction as a function of irradiation time: both activities are linearly related in UV-B-treated chloroplasts but nonlinearly in the case of treatment with UV-C radiation. If one accepts that UV-B and UV-C radiation transform PS II reaction centers into dissipative exciton sinks, the differences can be understood with the additional assumption that UV-B radiation interrupts exciton migration between different photosynthetic units, while this migration remains unaffected in UV-C-treated chloroplasts.

UV-C-treated leaves reveal no recovery of the variable fluorescence reflecting electron transport activity, even 5 days after irradiation (5 or 30 min duration).

Keywords

Reaction Center Photo System Photosynthetic Electron Transport Fluorescence Induction Variable Fluorescence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • G. Renger
    • 1
  • M. Voss
    • 1
  • P. Gräber
    • 1
  • A. Schulze
    • 1
  1. 1.Technische Universität BerlinFederal Republic of Germany

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