Application of Monoclonal Antibodies for Epidemiological Investigations and Oral Vaccination Studies
I. Monoclonal antibody analysis of bat origin virus isolates from South Africa revealed the parallel existence of Mokola and Duvenhage viruses among bats and of classical rabies virus among terrestrial animals of the same ecosystem. A Mokola type virus was causing clinical rabies in a cat from Natal, RSA.
Virus isolates from bats, from Maritime Germany (West) were shown to be identical to African origin Duvenhage virus.
II. An outbreak of rabies on a Norwegian island among mammals including one seal was shown by a particular nucleocapsid antibody to be caused by arctic fox rabies virus which according to this study seems to be restricted to the arctic circle.
III. A particular NC antibody identifying ERA/SAD viruses is presently being used by diagnostic laboratories in Germany and Switzerland to identify vaccine virus used in field trials for the oral immunization of foxes.
An antigenic variant of rabies virus selected by growing virus in the presence of a particular monoclonal antibody and found to be non-pathogenic for adult mice was applied orally to foxes by bait. The variant vaccine induced 100% seroconversion in foxes and proved to be at least equally protective as the vaccines used so far in Swiss and German field trials for the oral immunization of wildlife.
KeywordsVaccine Strain Rabies Virus Variant Virus Canine Rabies Rabies Virus Isolate
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