Application of Monoclonal Antibodies for Epidemiological Investigations and Oral Vaccination Studies
I. Monoclonal antibody analysis of bat origin virus isolates from South Africa revealed the parallel existence of Mokola and Duvenhage viruses among bats and of classical rabies virus among terrestrial animals of the same ecosystem. A Mokola type virus was causing clinical rabies in a cat from Natal, RSA.
Virus isolates from bats, from Maritime Germany (West) were shown to be identical to African origin Duvenhage virus.
II. An outbreak of rabies on a Norwegian island among mammals including one seal was shown by a particular nucleocapsid antibody to be caused by arctic fox rabies virus which according to this study seems to be restricted to the arctic circle.
III. A particular NC antibody identifying ERA/SAD viruses is presently being used by diagnostic laboratories in Germany and Switzerland to identify vaccine virus used in field trials for the oral immunization of foxes.
An antigenic variant of rabies virus selected by growing virus in the presence of a particular monoclonal antibody and found to be non-pathogenic for adult mice was applied orally to foxes by bait. The variant vaccine induced 100% seroconversion in foxes and proved to be at least equally protective as the vaccines used so far in Swiss and German field trials for the oral immunization of wildlife.
KeywordsEurope Agarose Titration Encephalitis Nigeria
Unable to display preview. Download preview PDF.
- 2.Schneider LG, Meyer S (1981) Antigenic determinants of rabies virus as demonstrated by monoclonal antibody. In: David HL, Bishop Compans RW (eds) The Replication of Negative Strand Viruses. Copyright 1981 by Elsevier North Holland, IncGoogle Scholar
- 7.Kantorovich RA (1964) Natural foci of a rabies-like infection in the far north. J Hyg Epid Microbiol Immunol 8:100–110Google Scholar
- 8.Schneider LG (1964) Erfahrungen mit fluoreszenzmarkierten Antikörpern bei der routinemäßigen Laboratoriumsdiagnose der Tollwut. I. Die fluoreszierende Antikörpertechnik. Zbl Vet Med 11:207–230Google Scholar
- 9.Smith JS, Yager PA, Baer GM (1973) A rapid tissue culture test for determining rabies neutralizing antibody. In: Kaplan MM, Koprowski H (third Edition) Laboratory Techniques in Rabies, pp 354–357Google Scholar
- 11.Barnard BJH, Hassel RH (1981) Rabies in Kudus (Tragelaphus Strepsiceros) in South West Africa/Namibia. J South Afric Vet Ass 52:309–314Google Scholar
- 14a.Crick J, Tignor GH, Moreno K (1981) Lagos bat virus in South Africa. CDC Rab Inf Exchange, June 1981, pp 40–41Google Scholar
- 18.Steck F, Wandeler A, Bichsel P, Capt S, Schneider LG (1982) Oral immunisation of foxes against rabies. A field study. Zbl Vet Med B 29:372–396Google Scholar
- 19.Schneider LG, Cox JH (1983) Ein Feldversuch zur oralen Immunisierung von Füchsen gegen die Tollwut in der Bundesrepublik Deutschland. I. Unschädlichkeit, Wirksamkeit und Stabilität der Vakzine SAD B19. Tierärztl Umschau 38:315–324Google Scholar