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Microbiological and Immunological Assays of Antimicrobials

Conference paper

Abstract

In antibiotic therapy, assays of serum (or other body fluids) from patients are performed for two principle reasons: to ensure that sufficient agent is present for adequate therapy and to monitor the patient for potentially toxic concentrations of the agent administered. Of the many methods of assay that are available, two, namely microbiological and immunological are mainly used in routine microbiological laboratories. In this paper I wish to examine the major advantages and drawbacks of the two methods.

Keywords

Latex Agglutination Microbiological Assay Immunological Assay Agar Diffusion Assay Serum Gentamicin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Andrews JM, Wise R (1984) A comparison of the homogeneous enzyme immunoassay and polarisation fluoroimmunoassay of gentamicin. J Antimicrob Chemother 14: 509–520PubMedCrossRefGoogle Scholar
  2. Broughall JM (1978) Aminoglycosides. In: Reeves DS, Phillips I, Williams JD, Wise R (eds) Laboratory methods in antimicrobial chemotherapy. Churchill Livingston, London, pp 194–207Google Scholar
  3. Noone P, Pattison JR, Sampson D (1971) Simple rapid method for assay of aminoglycoside antibiotics. Lancet I: 16–19CrossRefGoogle Scholar
  4. Reeves DS, Bywater MJ (1975) Quality control of serum gentamicin assays — experience of national surveys. J Antimicrob Chemother 1: 103–106PubMedCrossRefGoogle Scholar
  5. Reeves DS, Bywater MJ (1983) Comments on antibiotic quality control. Public Health Laboratory Service PublicationGoogle Scholar
  6. Shanson DS, Hince C (1977) Serum gentamicin and assays of 100 clinical serum samples by a rapid 40ºC Klebsiella method compared with overnight plate diffusion and acetyltransferase assay. J Clin Pathol 30: 521–525PubMedCrossRefGoogle Scholar
  7. Weber G (1953) Rotational brownian motion and polarisation of the fluorescence of solutions. Adv Protein Chemi 8: 415–419CrossRefGoogle Scholar
  8. Wills PJ, Wise R (1979) Rapid simple enzyme immunoassay for gentamicin. Antimicrob Agents Chemother 16: 40–42PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • R. Wise
    • 1
  1. 1.Department of MicrobiologyDudley Road HospitalBirminghamGreat Britain

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