Chromatographic Analysis for Identification of Microorganisms and Diagnosis of Infection: An Introduction
Since its introduction in 1905, chromatography has played an important role in microbiology. Over the years, microbiologists have used chromatographic procedures for isolation, purification, and separation of bacterial components, byproducts, and products from metabolism. Column and paper chromatography were the principal techniques used until the introduction of gas-liquid chromatography (GLC) and thin-layer chromatography (TLC) in the 1950s. The accuracy, speed, sensitivity, and simplicity of GLC and TLC resulted in a major expansion of chromatography to essentially all areas of microbiology.