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DNA Technology Applied to the Detection and Epidemiology of Enteric Pathogens

  • L. S. Tompkins
  • P. A. Mickelsen
  • N. Troup

Abstract

New techniques to isolate, clone, and manipulate DNA can now be applied to the detection of enteric pathogens in clinical materials, using specific gene sequences as probes. Although no DNA probes are yet commercially available, several have been developed and applied in research laboratories. In presenting our work on the development of a DNA probe for Campylobacter jejuni and its application to fecal samples, we will address the strategies which can be used in probe development and will outline the technical limitations which now exist in probing samples directly. In addition to using DNA as a diagnostic reagent, the plasmid or chromosomal DNA of enteric pathogens, including Campylobaeter and Salmonella, can be used as an epidemiological tool to differentiate strains of the same species. Preliminary data from experiments on Salmonella enteritidis will be presented.

Keywords

Chlamydia Trachomatis Neisseria Gonorrhoeae Enteric Pathogen Colony Hybridization Cryptic Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • L. S. Tompkins
  • P. A. Mickelsen
  • N. Troup
    • 1
  1. 1.Stanford University Medical Center S-156StanfordUSA

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