Abstract
A small subpopulation of splenic B cells carries the Ly-1 antigen, previously considered restricted to T cells (Hardy et al., 1982; Manohar et al.). The cells in this “Ly-1 B” subpopulation express other B lineage cell surface antigens, are detectable in spleen, are enriched in peritoneal washouts, are rare in (or absent from) lymph nodes and bone marrow and appear early during development (Hayakawa et al., 1983; Hayakawa et al., 1984). Thus Ly-1 B cells constitute a significant fraction of the Ig+ cells in spleens of newborn (3–5 days) mice, but decrease to a minor population (1–2 percent of total lymphocytes) in adults.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Braun J (1983) Spontaneous in vitro occurence and long-term culturing of murine B lymphoblastoid cell lines. J Immunol 130: 2113–2116
Coffman RL (1983) Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev 69: 5–23
Dangl JL, Herzenberg LA (1982) Selection of hybridomas and hybridoma variants by the fluorescence activated cell sorter. J Immunol Methods 52: 1–14
Davidson WF, Fredrickson TN, Rudikoff EK, Coffman RL, Hartley JW, Morse HC III (1984) A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J Immunol (submitted)
Eckhardt LA, Herzenberg LA (1980) Monoclonal antibodies to ThB detect close linkage of Ly-6 and a gene regulating ThB expression. Immunogenetics 11: 275–291
Hardy RR, Hayakawa K, Haaijman J, Herzenberg LA (1982) B cell subpopulations identifiable by two-color fluorescence analysis using a dual-laser FACS. In: Thorbecke GJ, Leslie GA (eds) Immunoglobulin D: Structure and Function (Annals of the New York Academy of Science, Vol. 399 ) p. 112
Hardy RR, Hayakawa K, Parks DR, Herzenberg LA (1983) Demonstration of B cell maturation in X-linked immunodeficient mice by simultaneous three-color immunofluorescence. Nature 306: 270–272
Hardy RR, Hayakawa K, Parks DR, Herzenberg LA, Herzenberg LA (1984) Murine B cell differentiation lineages. J Exp Med (in press)
Hayakawa K, Hardy RR, Parks DR, Herzenberg LA (1983) The “Ly-1 B” cell subpopulation in normal, immunodefective and autoimmune mice. J Exp Med 157: 202–218
Hayakawa K, Hardy RR, Herzenberg LA, Steinberg AD, Herzenberg LA (1984) Ly-1 B: A functionally distinct B cell subpopulation. In: Tada T (ed) Progress in Immunology (5th Internat’l. Congress of Immunology, Kyoto, Japan; August, 1983) Academic Press (in press)
Hayakawa K, Hardy RR, Honda M, Herzenberg LA, Steinberg AD, Herzenberg LA (1984) Ly-1 B cells: Functionally distinct lymphocytes that secrete IgM autoantibodies. Proc Nat’l Acad Sci USA (in press)
Kappler JW, Skidmore B, White J, Marrack P (1981) Antigen inducible, H-2-restricted interleukin-2-producing T cell hybridomas. J Exp Med 153: 1198–1214
Kincade PW, Lee G, Sun L, Watanabe T (1981) Monoclonal rat antibodies to murine IgM determinants. J Immunol Methods 42: 17–26
Lanier LL, Warner NL, Ledbetter JA, Herzenberg LA (1981) Expression of Lyt-1 antigen on certain murine B cell lymphomas. J Exp Med 153: 998–1003
Ledbetter JA, Herzenberg LA (1979) Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47: 63–90
Manohar VE, Brown E, Leiserson WM, Chused TM (1982) Expression of Lyt-1 by a subset of B lymphocytes. J Immunol 129: 532–538
Oi VT, Jones PP, Goding JW, Herzenberg LA, Herzenberg LA (1978) Properties of monoclonal antibodies to mouse Ig allotypes, H-2 and la antigens. Current Topics in Microbiol and Immunol 81: 115–129
Parks DR, Hardy RR, Herzenberg LA (1984) Three-color immunofluorescence analysis of mouse B lymphocyte subpopulations. Cytometry (in press)
Springer T, Galfre G, Secher DS, Milstein C (1979) Mac-1: a macrophage differentiation antigen identified by a monoclonal antibody. Eur J Immunol 9: 301–306
Whitlock CA, Witte ON (1982) Long term culture of B lymphocytes and their precursors from murine bone marrow. Proc Nat’l Acad Sci USA 79: 3608–3612
Yelton DE, Desaymard C, Scharff MD (1981) Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma 1: 5–11
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer-Verlag Berlin · Heidelberg
About this chapter
Cite this chapter
Hardy, R.R., Hayakawa, K., Herzenberg, L.A., Morse, H.C., Davidson, W.F. (1984). Ly-1 as a Differentiation Antigen on Normal and Neoplastic B Cells. In: Potter, M., Melchers, F., Weigert, M. (eds) Oncogenes in B-Cell Neoplasia. Current Topics in Microbiology and Immunology, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69860-6_39
Download citation
DOI: https://doi.org/10.1007/978-3-642-69860-6_39
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-69862-0
Online ISBN: 978-3-642-69860-6
eBook Packages: Springer Book Archive