Summary
Several DNAase I hypersensitive sites mark the putative regulatory region immediately 5′ of the myc gene. A sequence near at least one site binds to a protein(s) from nuclear extracts in vitro. Three patterns of chromatin structure exist, one associated with the translocated myc allele in a Burkitt lymphoma (BL 31), one associated with the non-translocated (germline) allele in the same Burkitt cell and one associated with the germline myc allele in non-malignant B cells. The non-translocated and transcriptionally silent myc allele in BL 31 shows only one strong hypersensitive site, a site which may mediate negative control over myc. The heavy chain immunoglobulin enhancer that is juxtaposed with myc on the translocated allele in BL 31 may be responsible for the chromatin structure on this deregulated allele. These data have novel implications for the activation of the myc oncogene by translocations.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Battey J, Moulding C, Taub R, Murphy W, Stewart T, Potter H, Lenoir G, Leder P (1983) The human c-myc oncogene: structural consequences of translocation into the IgH in Burkitt lymphoma. Cell 34: 779–789
Bernard O, Cory S, Gerondakis S, Webb E, Adams JM (1983) Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumors. EMBO J 2: 2375–2383
Dalla-Favera R, Martinotti S, Gallo R, Erikson J, Croce C (1983) Translocation and rearrangments of the c-myc oncogene locus in human undifferentiated B-cell lymphomas. Science 219: 963–967
Dierks P, van Ooyen A, Cochran M, Dobkin C, Reiser J, Weissmann C (1983) Three regions upstream from the CAP site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells. Cell 32: 695–706
Emerson BM, Felsenfeld G (1984) Specific factor conferring nuclease hypersensitivity at the 5′ end of the chicken adult beta-globin gene. Proc Natl Acad Sci USA 81: 95–99
Erikson J, ar-Rushdi A, Drwinga HL, Nowell PC, Croce C (1983) Transcriptional activation of the translocated c-myc oncogene in Burkitt lymphoma. Proc Natl Acad Sci USA 80: 820–824
Hamlyn PH, Rabbitts TH (1983) Translocation joins c-myc and immunoglobulin gamma-1 genes in a Burkitt lymphoma revealing a third exon in the c-myc oncogene. Nature 304: 135–139
Hayday A, Gillies S, Saito H, Wood C, Wiman K, Hayward W, Tonegawa S (1984) Activation of a translocated human c-myc gene by an enhancer in the immunoglobulin heavy chain locus. Nature 307: 334–340
Kelly K, Cochran B, Stiles C, Leder P (1983) Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet derived growth factor. Cell 35: 603–610
Leder P, Battey J, Lenoir G, Moulding C, Murphy W, Potter H, Stewart T, Taub R (1983) Translocations among antibody genes in human cancer. Science 222: 765–771
Maguire RT, Robins TS, Thorgeirsson S, Heilman CA (1983) Expression of cellular myc and mos genes in undifferentiated B cell lymphomas of Burkitt and non-Burkitt types. Proc Natl Acad Sci USA 80: 1947–1950
Mills F, Fisher M. Kuroda R, Ford A, and Gould H (1983) DNAase I hypersensitive sites in the chromatin of human mu Immunoglobulin heavy-chain genes. Nature 306: 809–812.
Nagata K, Guggenheimer RA, Hurwitz J (1983) Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA. Proc Natl Acad Sci USA 80: 6177–6181
Nishikura K, ar-Rushdi A, Erikson J, Watt R, Rovera G, Croce CM (1983) Differential expression of the normal and of the translocated human c-myc oncogenes in B cells. Proc Natl Acad Sci USA 80: 4822–4826
Parslow T, Granner D (1983) Structure of a nuclease-sensitive region inside the immunoglobulin kappa gene: evidence for a role in gene regulation. Nucl Acids Res 11: 4775–4792
Rabbitts TH, Hamlyn PH, Baer R (1983) Altered nucleotide sequence of a translocated c-myc gene in Burkitt lymphoma. Nature 306: 760–765
Schubach W, Groudine M (1984) Alteration of c-myc chromatin structure by avian leukosis virus integration. Nature 307: 702–708.
Siebenlist U, Hennighausen L, Battey J, Leder P (1984) Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt lymphoma. Cell, submitted.
Stalder J, Larsen A, Engel J, Dolan M, Groudine M, Weintraub H (1980) Tissue- specific DNA cleavages in the globin chromatin domain introduced by DNAase I. Cell 20: 451–460
Stanton LW, Watt R, Marcu KB (1983) Translocation, breakage and truncated transcripts of c-myc oncogene in murine plasmacytomas. Nature 303: 401–406
Taub R, Kirsch I, Morton C, Lenoir G, Swan D, Tronick S, Aaronson S, Leder P (1982) Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc Natl Acad Sci USA 79: 7837–7841
Taub R, Moulding C, Battey J, Murphy W, Vasicek T, Lenoir G, Leder P (1984) Activation and somatic mutation of the translocated c-myc gene in Burkitt lymphoma cells. Cell 36: 339–348
Westin EH, Wong-Staal F, Gelman EP, Dalla-Favera R, Papas T, Lautenberger JA, Eva A, Reddy EP, Tronick SR, Aaronson SA, Gallo RC (1982) Expression of cellular homologs of retroviral one genes in human hematopoietic cells. Proc Natl Acad Sci USA 79: 2490–2494
Wu C (1980) The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNAase I. Nature 286: 854–860
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer-Verlag Berlin · Heidelberg
About this chapter
Cite this chapter
Siebenlist, U., Hennighausen, L., Battey, J., Leder, P. (1984). Chromatin Structural Changes in the Putative Regulatory Region of c-myc Accompany the Translocation in a Burkitt Lymphoma. In: Potter, M., Melchers, F., Weigert, M. (eds) Oncogenes in B-Cell Neoplasia. Current Topics in Microbiology and Immunology, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69860-6_31
Download citation
DOI: https://doi.org/10.1007/978-3-642-69860-6_31
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-69862-0
Online ISBN: 978-3-642-69860-6
eBook Packages: Springer Book Archive