Abstract
The polymorphism of the red cell enzyme glyoxalase I (GLO-I), first described by Kömpf et al. [1] using starch gel electrophoresis, was investigated by isoelectric focusing (IEF) on polyacrylamide plates at pH 4-5 (LKB, PAG plates). Briefly, 20 μl red cell lysate was applied near the cathode. Following the focusing, the gels were incubated for 20 minutes at 37 °C in a 0.2 M phosphate buffer containing 257 mM methylglyoxal and 16.3 mM reduced glutathione. The detection gel consisted of 1% agarose in 0.1 M Tris buffer with 0.06 mM DCIP and 2.4 mM MTT poured on a GelBond film.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Kömpf J, Bissbort S, Gussman S, Ritter H (1975) Polymorphism of a new genetic marker in man. Hum Genet 27: 141–143
Rittner C, Weber W (1978) Evidence for a “silent allele” Glo° at the glyoxalase I locus. Hum Genet 42: 315–318
Rubinstein P, Suciu-Foca N (1979) Glyoxalase I: a possible “null” allele. Hum Hered 29: 217–220
Sparkes RS, Sparkes MC, Crist M, Anderson CE (1983) Glyoxalase I “null” allele in a new family. Hum Genet 64: 146 - 147
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Klouda, P.T., Bidwell, J.L. (1984). Glyoxalase I (GLO I) Phenotyping by Isoelectric Focusing. In: Albert, E.D., et al. Histocompatibility Testing 1984. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69770-8_220
Download citation
DOI: https://doi.org/10.1007/978-3-642-69770-8_220
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-69772-2
Online ISBN: 978-3-642-69770-8
eBook Packages: Springer Book Archive