Isolation and Characterization of the cDNA Clones and the Genomic Clones of the HLA Class II Antigen Heavy Chains
The class II HLA antigens, Ia antigens, are essential for cellular recognition and cooperation in immune response [1, 2]. A cluster of genes encoding these class II antigens has been mapped in the HLA-D region of human chromosome 6. Serological as well as cellular reagents allowed the identification of two sets of molecular entities that display a similar function and tissue distribution to HLA-D products. These antigens, named DR and DQ, are composed of two noncovalently associated glycosylated 33 000 dalton α (heavy) and 28 000 dalton β (light) chains. The latter carries the main polymorphic determinants. Recently, another human class II antigen, named DP, has been defined by cellular assay which is encoded in subregions distinct from DR and DQ [3, 4]. DP antigens control a secondary lymphocyte reaction and are encoded in a region centromic to the DR loci. Further, biochemical data and genetic approach with cloned DNA probes provide evidence for several other distinct α and β chains of human Ia molecules [5–9].
KeywordsSucrose Codon Acid Number Proline Tryptophan
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