Abstract
Complete cerebral ischemia causes cytotoxic brain edema, and eventually irreversible cell death in central nervous tissue (3, 7). The mechanisms causing swelling of nerve and glial elements, and finally cell destruction are far from being understood, however. Up to now, anoxia is believed to induce a breakdown of intra/extracellular ion gradients with a concomitant cell hydrops on account of a failing energy supply to the active ion pumps. On the other hand, there is growing evidence that additional mechanisms are involved in anoxia, such as a release of glutamate, K+ ions, free fatty acids, free radicals, etc. (1). We have introduced an in vitro model to examine the mechanisms of cytotoxic cell swelling of nerve and glial cells by monitoring cell volume changes of viable cells under strictly controlled conditions (6).
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Zimmer, M., Kempski, O., Neu, A., von Rosen, F., Baethmann, A. (1984). Anoxic Incubation of Suspended Glial Cells. An In-Vitro Model of Cerebral Anoxia to Study Cytotoxic Brain Edema. In: Piotrowski, W., Brock, M., Klinger, M. (eds) CNS Metastases Neurosurgery in the Aged. Advances in Neurosurgery, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69360-1_46
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DOI: https://doi.org/10.1007/978-3-642-69360-1_46
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