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High-Affinity Monoclonal Antibodies Specific for Deoxynucleosides Structurally Modified by Alkylating Agents: Applications for Immunoanalysis

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Book cover Biological Dosimetry

Abstract

Mutagenic and carcinogenic chemicals, most cancer chemotherapeutic agents, as well as ionizing radiation and ultraviolet light, cause structural alterations of DNA in the chromatin of target cells (see Town et al. 1973; Lawley 1976; Wang 1976; Grover 1979; Rajewsky 1980; Waring 1981; Singer and Kuśmierek 1982). In the case of alkylating chemical agents, covalent binding generally occurs between nucleophilic centers (electron-rich N and О atoms) in DNA and strongly reactive, electrophilic derivatives, generated from the respective parent compounds either enzymatically (“metabolic activation”) or via non-enzymatic decomposition (see Magee 1974; Miller and Miller 1979, 1981; Gelboin 1980). As for most of the DNA alterations caused by various types of radiation, the precise structure of many chemical DNA adducts remains to be elucidated. Nevertheless, several classes of DNA-reactive chemicals have already been very thoroughly studied with regard to their DNA binding products, and in relation to the potential consequences of these DNA adducts in terms of cytotoxicity, mutagenesis, or malignant transformation. Thus, the various types of structural modifications of DNA resulting from the exposure of cells to alkylating N-nitroso compounds (alkylnitrosoureas, al-kylnitrosoamines, alkylnitro-nitroso-guanidines) have for the most part been identified (Lawley 1976; Grover 1979; Singer 1979; Rajewsky 1980, 1983).

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Authors and Affiliations

  1. Institut für Zellbiologie (Tumorforschung), Universität Essen (GH), Hufelandstrasse 55, D-4300, Essen 1, Germany

    J. Adamkiewicz, O. Ahrens & M. F. Rajewsky

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  1. J. Adamkiewicz
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  2. O. Ahrens
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  3. M. F. Rajewsky
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Editors and Affiliations

  1. Arbeitsgruppe Zytometrie, Gesellschaft für Strahlen- und Umweltforschung mbH, Herrenhäuser Straße 2, 3000, Hannover 21, Germany

    Wolfgang G. Eisert

  2. Biologische Forschung, Dr. K. Thomae GmbH, 7950, Biberach-Riss, Germany

    Wolfgang G. Eisert

  3. Biomedical Sciences Division, Lawrence Livermore National Laboratory, P.O. Box 5570, 94550, Livermore, CA, USA

    Mortimer L. Mendelsohn

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© 1984 Springer-Verlag Berlin Heidelberg

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Adamkiewicz, J., Ahrens, O., Rajewsky, M.F. (1984). High-Affinity Monoclonal Antibodies Specific for Deoxynucleosides Structurally Modified by Alkylating Agents: Applications for Immunoanalysis. In: Eisert, W.G., Mendelsohn, M.L. (eds) Biological Dosimetry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69334-2_30

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  • DOI: https://doi.org/10.1007/978-3-642-69334-2_30

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