Culture of Human Lung Carcinoma Cells in Serum-Free Media
In this article, we describe methods for serum-free culture of human lung carcinoma cells. The original tumors were obtained from surgery and the tumors have been successively transplanted into nude mice. We have studied the growth requirements of these tumor cells in primary cultures and of established cell lines. Each tumor type can be grown as transplantable tumors, either in nude mice or in culture, allowing in vivo and in vitro experiments. We have developed serum-free media for these cell lines, which allow us to examine the biological effects of many substances.
KeywordsNude Mouse Retinoic Acid Primary Culture Established Cell Line Growth Requirement
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