Culture of Human Lung Carcinoma Cells in Serum-Free Media

  • Hideo Masui
  • Kaoru Miyazaki
  • Gordon H. Sato
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

In this article, we describe methods for serum-free culture of human lung carcinoma cells. The original tumors were obtained from surgery and the tumors have been successively transplanted into nude mice. We have studied the growth requirements of these tumor cells in primary cultures and of established cell lines. Each tumor type can be grown as transplantable tumors, either in nude mice or in culture, allowing in vivo and in vitro experiments. We have developed serum-free media for these cell lines, which allow us to examine the biological effects of many substances.

Keywords

Albumin Adenocarcinoma Hydrocortisone Selenium Dodecyl 

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References

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    Miyazaki, K., Masui, H. and Sato, G. Control of keratinization of human broncogenic epidermoid carcinoma cells in primary culture with serum-free medium. Proc. Natl. Acad. Sci. USA, in press (1982).Google Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • Hideo Masui
    • 1
  • Kaoru Miyazaki
    • 1
  • Gordon H. Sato
    • 1
  1. 1.Cancer Center, Q-058University of CaliforniaSan Diego, La JollaUSA

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