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Characterization of Cardiomyocytes Cultured in Serum-Free Medium

  • Gania Kessler-Icekson
  • Lina Wasserman
  • Ester Yoles
  • S. R. Sampson
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Primary cultures of rat cardiomyocytes were grown in serum-free defined medium. Comparison between these cells and cells grown in the presence of serum has shown that myocytes of both cultures are similar in many aspects and share a number of cardiac specific features. Cardiomyocytes in either medium maintained their beating capacity throughout the culture period (2–3 weeks). Mean transmembrane resting potentials recorded from beating cells grown in defined or complete medium were -77mV and -80mV respectively. Glycogen accumulation and myofibril formation were clearly demonstrated in both types of culture. A cleaner myocytic population was obtained in the defined medium due to reduced proportion of non-myocytes.

Keywords

Glycogen Accumulation Action Potential Record Spontaneous Beating Phase Contrast Photomicrography Hepes 10mM 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • Gania Kessler-Icekson
    • 1
  • Lina Wasserman
    • 1
  • Ester Yoles
    • 2
  • S. R. Sampson
    • 2
    • 3
  1. 1.The Rogoff-Wellcome, Medical Research InstituteBar Ilan UniversityRamat GanIsrael
  2. 2.Beilinson Medical Center, Petah-Tikva and the Department of Life SciencesBar Ilan UniversityRamat GanIsrael
  3. 3.Department of Experimental PhysiologyBar Ilan UniversityRamat GanIsrael

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