Characterization of Cardiomyocytes Cultured in Serum-Free Medium
Primary cultures of rat cardiomyocytes were grown in serum-free defined medium. Comparison between these cells and cells grown in the presence of serum has shown that myocytes of both cultures are similar in many aspects and share a number of cardiac specific features. Cardiomyocytes in either medium maintained their beating capacity throughout the culture period (2–3 weeks). Mean transmembrane resting potentials recorded from beating cells grown in defined or complete medium were -77mV and -80mV respectively. Glycogen accumulation and myofibril formation were clearly demonstrated in both types of culture. A cleaner myocytic population was obtained in the defined medium due to reduced proportion of non-myocytes.
KeywordsFormalin Hydrocortisone Trypsin Hepes Hematoxylin
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