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Hormonally Defined Media pp 337–339Cite as

Isolation and Characterization of MDCK-Subcultures

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Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

In order to obtain uniform populations derived from the MDCK cell line, the technique of centrifugal elutriation has been used. Seven cell types, which differ from each other by their size have been subcultured and characterized with regards to their morphology and stimulation of adenylate cyclase. The latter is inducible by isoproterenol in a rapid and dose dependent way. The smallest cells are the most sensitive to isoproterenol; the increase of enzyme activity brought about by these cells is higher than that of the original cell line.

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Author information

Authors and Affiliations

  1. Institut de Pharmacologie, Faculté de Médecine, F-67000, Strasbourg, France

    E. M. Giesen, C. Welsch, M. Schmidt, J. L. Imbs & J. Schwartz

  2. Institut de Virologie, Faculté de Médecine, F-67000, Strasbourg, France

    A. Steffan

Authors
  1. E. M. Giesen
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  2. C. Welsch
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  3. M. Schmidt
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  4. J. L. Imbs
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  5. A. Steffan
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  6. J. Schwartz
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Editor information

Editors and Affiliations

  1. Institut für Neurobiologie, Universität Heidelberg, Im Neuenheimer Feld 504, 6900, Heidelberg 1, Germany

    G. Fischer

  2. Institut für Pharmakologie, Universität Mainz, Obere Zahlbacher Str. 67, 6500, Mainz, Germany

    R. J. Wieser

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© 1983 Springer-Verlag Berlin Heidelberg

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Giesen, E.M., Welsch, C., Schmidt, M., Imbs, J.L., Steffan, A., Schwartz, J. (1983). Isolation and Characterization of MDCK-Subcultures. In: Fischer, G., Wieser, R.J. (eds) Hormonally Defined Media. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69290-1_45

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  • DOI: https://doi.org/10.1007/978-3-642-69290-1_45

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69292-5

  • Online ISBN: 978-3-642-69290-1

  • eBook Packages: Springer Book Archive

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