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Isolation and Characterization of MDCK-Subcultures

  • E. M. Giesen
  • C. Welsch
  • M. Schmidt
  • J. L. Imbs
  • A. Steffan
  • J. Schwartz
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

In order to obtain uniform populations derived from the MDCK cell line, the technique of centrifugal elutriation has been used. Seven cell types, which differ from each other by their size have been subcultured and characterized with regards to their morphology and stimulation of adenylate cyclase. The latter is inducible by isoproterenol in a rapid and dose dependent way. The smallest cells are the most sensitive to isoproterenol; the increase of enzyme activity brought about by these cells is higher than that of the original cell line.

Keywords

Adenylate Cyclase Toluidine Blue MDCK Cell cAMP Content Uniform Population 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • E. M. Giesen
    • 1
  • C. Welsch
    • 1
  • M. Schmidt
    • 1
  • J. L. Imbs
    • 1
  • A. Steffan
    • 2
  • J. Schwartz
    • 1
  1. 1.Institut de Pharmacologie, Faculté de MédecineStrasbourgFrance
  2. 2.Institut de Virologie, Faculté de MédecineStrasbourgFrance

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