Summary
A short review of the current status of enzyme isolation and purification by liquid-liquid extraction in aqueous two-phase systems is given. The method can be used mainly for the separation of the desired enzyme or also protein in general from cell debris, contaminating proteins including interfering activities, nucleic acids and polysaccharides. Working on large scale phase separation is performed by commercially available separators or in settling tanks. So far, more than 20 enzymes were separated this way from broken cells of procaryotic and eucaryotic microorganisms. Enzyme yields were usually in the range of 90–100% and the purification factor related to removal of contaminating protein varied between 1 and 8. A number of those enzymes were further purified by subsequent partition steps. As a special example, the extractive purification of aspartase from E. coli is discussed here.
Furthermore, a general scheme for extractive enzyme purification is derived taking into account several parameters important for the process design. In the last chapter continuous enzyme isolation by liquid-liquid extraction is discussed.
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© 1983 Springer-Verlag, Berlin, Heidelberg
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Hustedt, H., Kroner, K.H., Schütte, H., Kula, MR. (1983). Extractive Purification of Enzymes. In: Lafferty, R.M. (eds) Enzyme Technology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69148-5_13
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DOI: https://doi.org/10.1007/978-3-642-69148-5_13
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-12479-5
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