Advertisement

Role of Calcium and Cyclic Nucleotides in the Regulation of Intestinal Ion Transport

  • M. C. Rao
  • M. Field
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Studies in mammalian intestine reveal that a variety of humoral, microbial and pharmacological agents known to alter ion transport (either in the absorptive or secretory direction) appear to do so by altering the intracellular concentrations of calcium, cyclic AMP, or cyclic GMP (Field 1981). An increase in the intracellular concentration of any one of these mediators results in net secretion, whereas a decrease results in net absorption. Mammalian ileum can both absorb and secrete water and electrolytes. Net secretion can result from inhibition of an active absorptive process and/or the stimulation of an active secretory process. In contrast, the intestine of the marine teleost, Pseudopleuronectes americanus (winter flounder), only absorbs. This intestine is also devoid of crypts, which are believed to be the site of fluid secretion in mammalian intestine.

Keywords

Cyclic Nucleotide Intracellular Mediator Transepithelial Potential Difference Rabbit Ileum Mammalian Intestine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bolton J, Field M (1977) Ca-ionophore-stimulated ion secretion in rabbit ileal mucosa: relation to actions of cyclic AMP and carbamylcholine. J Membr Biol 35:159–174PubMedCrossRefGoogle Scholar
  2. Dietz J, Field M (1973) Ion transport in rabbit ileal mucosa. IV. Bicarbonate secretion. Am J Physiol 225:858–861PubMedGoogle Scholar
  3. Donowitz M, Battisti L, Madara JL, Trier JS, Cusolito S, Carlson S, Field M (1981) Calcium and active transport in intestine of the winter flounder, Pseudopleuronectes americanus. Bull Mt Desert Isl Biol Lab 21:22–26Google Scholar
  4. Duffey ME, Hainau B, Ho S, Bentzel CJ (1981) Regulation of epithelial tight junction permeability by cyclic AMP. Nature 294:451–453PubMedCrossRefGoogle Scholar
  5. Field M (1971) Ion transport in rabbit ileal mucosa. II. Effects of cyclic 3′,5′-AMP. Am J Physiol 221:992–997PubMedGoogle Scholar
  6. Field M (1978) Some speculations on the coupling between sodium and chloride transport processes in mammalian and teleost intestine. In: Hoffman JF (ed) Membrane transport processes, vol I. Raven Press, New York, pp 277–292Google Scholar
  7. Field M (1981) Secretion of electrolytes and water by mammalian small intestine. In: Johnson LR (ed) Physiology of the gastrointestinal tract. Raven Press, New York, pp 963–982Google Scholar
  8. Field M, Graf LH, Laird WJ, Smith PL (1978a) Heat stable enterotoxin of Escherichia coli: in vitro effects of guanylate cyclase activity, cyclic GMP concentration and ion transport in small intestine. Proc Natl Acad Sci USA 75:2800–2804PubMedCrossRefGoogle Scholar
  9. Field M, Karnaky KJ, Smith PL, Bolton JE, Kinter WB (1978b) Ion transport across the isolated intestinal mucosa of the winter flounder, Pseudopleuronectes americanus. I. Functional and structural properties of cellular and paracellular pathways for Na and CI. J Membr Biol 41: 265–293PubMedCrossRefGoogle Scholar
  10. Field M, Smith PL, Bolton JE (1980) Ion transport across the isolated intestinal mucosa of the winter flounder, Pseudopleuronectus americanus. II. Effects of cyclic AMP. J Membr Biol 55: 157–163PubMedCrossRefGoogle Scholar
  11. Frizzell RA (1977) Active chloride secretion by rabbit colon: calcium-dependent stimulation by ionophore A23187. J Membr Biol 35:175–187PubMedCrossRefGoogle Scholar
  12. Frizzell RA, Dugas MC, Schultz SG (1975) Sodium chloride transport by rabbit gall bladder. Direct evidence for a coupled NaCl influx process. J Gen Physiol 65:769–795PubMedCrossRefGoogle Scholar
  13. Frizzell RA, Field M, Schultz SG (1979a) Sodium-coupled chloride transport by epithelial tissues. Am J Physiol 236:F1-F8PubMedGoogle Scholar
  14. Frizzell RA, Smith PL, Vosburgh E, Field M (1979b) Coupled sodium-chloride influx across brush border of flounder intestine. J Membr Biol 46:27–39PubMedCrossRefGoogle Scholar
  15. Guandalini S, Rao MC, Smith PL, Field M (1982a) cGMP modulation of ileal ion transport: in vitro effects of Escherichia coli heat-stable enterotoxin. Am J Physiol 243:G36 G41PubMedGoogle Scholar
  16. Guandalini S, Migliavacca M, Campora E de, Rubino A (1982b) Cyclic guanosine monophosphate effects on nutrient and electrolyte transport in rabbit ileum. Gastroenterology 83:15–21PubMedGoogle Scholar
  17. Hayes JS, Brunton LL (1982) Functional compartments in cyclic nucleotide action. J Cyclic Nucl Res 8:1–16Google Scholar
  18. Ilundain A, Naftalin RJ (1979) Role of Ca2+-dependent regulator protein in intestinal secretion. Nature 279:446–448PubMedCrossRefGoogle Scholar
  19. Jonge HR de (1975) The localization of guanylate cyclase in rat small intestinal epithelium. FEBS Lett 53:237–242PubMedCrossRefGoogle Scholar
  20. Klyce SD, Wong RKS (1977) Site and mode of adrenaline action on chloride transport across the rabbit corneal epithelium. J Physiol (Lond) 266:777–799Google Scholar
  21. Krasny EJ Jr, Frizzell RA (1982) Regulation of paracellular perm selectivity in flounder intestine. Bull Mt Desert Isl Biol Lab 22: (in press)Google Scholar
  22. Leitch GJ, Burrows W (1968) Experimental cholera in the rabbit ligated intestine: ion and water accumulation in the duodenum, ileum and colon. J Infect Dis 118:349–359PubMedCrossRefGoogle Scholar
  23. Machan TE, Diamond JE (1969) An estimate of the salt concentration in the lateral intercellular spaces of rabbit gall bladder during maximal fluid transport. J Membr Biol 1:194–213CrossRefGoogle Scholar
  24. Musch MW, Orellana SA, Kimberg LS, Field M, Halm DR, Krasny EJ, Frizzell RA (1982a) Na/K/CI cotransport in the intestine of a marine teleost. Nature 300:351–353PubMedCrossRefGoogle Scholar
  25. Musch MW, Frizzell RA, Field M (1982b) Kinetics of Na, K, CI cotransport in flounder intestine. Bull Mt Desert Isl Biol Lab 22: (in press)Google Scholar
  26. Rao MC, Nash NT, Field M (1982) Cyclic GMP: the authentic inhibitor of Na, K, CI cotransport in flounder intestine. Bull Mt Desert Isl Biol Lab 22: (in press)Google Scholar
  27. Sheerin HE, Field M (1975) Ileal HC03 secretion: relationship to Na and CI transport and effect of theophylline. Am J Physiol 228:1065–1074PubMedGoogle Scholar
  28. Shorofsky S, Field M, Fozzard H (1982) Electrophysiology of CI secretion in canine trachea. J Membr Biol (in press)Google Scholar
  29. Silva P, Stoff JS, Field M, Fine L, Forrest JN, Epstein FH (1978) Mechanism of active chloride secretion by shark rectal gland: role of Na-K-ATPase in electrogenic chloride secretion. Am J Physiol 232:F298-F306Google Scholar
  30. Smith PL, Field M (1980) In vitro antisecretory effects of trifluoperazine and other neuroleptics in rabbit and human small intestine. Gastroenterology 78:1545–1553PubMedGoogle Scholar
  31. Smith PL, Blumberg JB, Stoff JS, Field M (1981) Antisecretory effects of indomethacin on rabbit ileal mucosa in vitro. Gastroenterology 80:356–365PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • M. C. Rao
  • M. Field
    • 1
    • 2
  1. 1.Departments of Medicine and of Pharmacological and Physiological SciencesThe University of ChicagoChicagoUSA
  2. 2.The Mount Desert Island Biological LaboratorySalsbury CoveUSA

Personalised recommendations