A Novel Non-Centrifugation Radioimmunoassay for Cannabinoids

Cais, M.; Dani, S.; Shimoni, M.

doi:10.1007/978-3-642-69083-9_15

M. Cais³,
S. Dani⁴ &
M. Shimoni⁵

Part of the book series: Archives of Toxicology ((TOXICOLOGY,volume 6))

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3 Citations

Abstract

A new method is described for the separation of free and bound fractions in a cannabinoid (³H)-radioimmunoassay without the need for centrifugation.

The method employs a novel separation device which allows all manipulations to be performed in a single tube simply, rapidly and safely.

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References

Cais M, Shimoni M (1981a) A feasible solvent separation system for immunoassays. Ann Clin Biochem 18: 317–323
CAS Google Scholar
Cais M, Shimoni M (1981b) A novel system for mass transport through selective barriers in non-centrifugation immunoassays. Ann Clin Biochem 18: 324–329
CAS Google Scholar
Cais M, Dani S, Josephy Y, Modiano A, Gershon H, Mechoulam R (1975) Studies of cannabinoid metabolites — a free-radical immunoassay. FEBS Lett 55: 257–260
Article PubMed CAS Google Scholar
Mechoulam R (ed) (1973) Marijuana, chemistry, pharmacology, metabolism and clinical effects. Academic Press, New York
Google Scholar
Odell WD, Silver C, Grover PK (1975) Competitive protein binding assays. Methods of separation of bound from free. In: Cameron EHD, Hillier SG, Griffith K (eds) Steroid immunoassays. Alpha Omega, Cardiff., pp 207–222
Google Scholar
Teale JD, Clough JM, King LJ, Marks V, Williams PL, Moffat AC (1979) Antisera raised against tetrahydrocannabinol in the radioimmunoassay of cannabinoids. In: Vinson JA (ed) Cannabinoid analysis in physiological fluids. American Chemical Society, Washington, pp 155–173
Chapter Google Scholar

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Authors and Affiliations

Department of Chemistry, Technion-Israel Institute of Technology, Haifa, 32000, Israel
M. Cais
Isler Clinical Toxicology Unit, The Chaim Sheba Medical Center, Tel-Hashomer, 52621, Israel
S. Dani
Lidex Corporation Ltd., Gutwirth Science-based Industries Center, Technion City, Haifa, 32000, Israel
M. Shimoni

Authors

M. Cais
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S. Dani
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M. Shimoni
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Editor information

Editors and Affiliations

Department of Pharmacology, Trinity College, University of Dublin, Dublin 2, Ireland
Claire M. Chambers & Philip L. Chambers &
Sackler Faculty of Medicine, and Kupat Holim — General Federation of Labour, Institute for Occupational Health, Tel Aviv University, Tel Aviv, Israel
S. Gitter

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Cais, M., Dani, S., Shimoni, M. (1983). A Novel Non-Centrifugation Radioimmunoassay for Cannabinoids. In: Chambers, C.M., Chambers, P.L., Gitter, S. (eds) Toxicology in the Use, Misuse, and Abuse of Food, Drugs, and Chemicals. Archives of Toxicology, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69083-9_15

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DOI: https://doi.org/10.1007/978-3-642-69083-9_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-12392-7
Online ISBN: 978-3-642-69083-9
eBook Packages: Springer Book Archive

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