Detection of Viral Antigens by Time-Resolved Fluoroimmunoassay
Highly sensitive immunoassays have proved to be valuable in the diagnosis of viral infections because viral antigens can be directly detected in clinical specimens by these assays. This technology was first widely used in the radioimmunoassay of hepatitis B surface antigen in serum (Ling and Overby 1972) and later applied to the detection of gastroenteritis viruses in stool (Halonen and Meurman 1982). Our group has also developed assays for the detection of seven respiratory viruses in nasopharyngeal secretions (Sarkkinen et al. 1981a-c), and these assays have now been successfully applied on a routine basis in our diagnostic laboratory. Immuno-assays are now widely used in the detection of antiviral antibodies and particularly in the assay of IgM antibodies.
KeywordsRespiratory Syncytial Virus Respiratory Virus Stool Specimen Polystyrene Bead Acute Respiratory Disease
Unable to display preview. Download preview PDF.
- Ekins RP (1981) The “precision profile”: its use in RIA assessment and design. Ligand Q 4: 33–44Google Scholar
- Gardner PS, McQuillin J (1974) Rapid virus diagnosis. Application of immunofluorescence. Butter-worths, LondonGoogle Scholar
- Halonen P, Meurman O (1982) Radioimmunoassay in diagnostic virology. In: Howard C (ed) New developments in practical virology. Liss, New York, pp 83–124Google Scholar
- Meurman OH, Hemmilä IA, Lövgren TN, Halonen PE (to be published) Time-resolved fluoro-immunoassay, a new test for rubella serology. J Clin MicrobiolGoogle Scholar
- Sarkkinen HK, Halonen PE, Arstila PP, Salmi AA (1981a) Detection of respiratory syncytial, parainfluenza type 2, and adenovirus antigens by radioimmunoassay and enzyme immunoassay on nasopharyngeal specimens from children with acute respiratory disease. J Clin Microbiol 13: 258–265PubMedGoogle Scholar