Detection of Viral Antigens by Time-Resolved Fluoroimmunoassay
Highly sensitive immunoassays have proved to be valuable in the diagnosis of viral infections because viral antigens can be directly detected in clinical specimens by these assays. This technology was first widely used in the radioimmunoassay of hepatitis B surface antigen in serum (Ling and Overby 1972) and later applied to the detection of gastroenteritis viruses in stool (Halonen and Meurman 1982). Our group has also developed assays for the detection of seven respiratory viruses in nasopharyngeal secretions (Sarkkinen et al. 1981a-c), and these assays have now been successfully applied on a routine basis in our diagnostic laboratory. Immuno-assays are now widely used in the detection of antiviral antibodies and particularly in the assay of IgM antibodies.
KeywordsHepatitis Titration Influenza Polystyrene Macromolecule
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