Abstract
Electrofocusing separates ampholytes such as proteins according to their isoelectric point. Different versions of the technique have been described under various unwieldy names such as isoelectric focusing or non-equilibrium pH-gradient electrophoresis, but we prefer to apply the term electrofocusing to any type of pH-gradient electrophoresis. In this paper we are concerned specifically with electrofocusing in polyacrylamide gels containing 9M urea. Under these conditions the protein subunits that make up biological structures can be separated as individual polypeptides with very high resolution.
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© 1983 Springer-Verlag Berlin Heidelberg
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King, A.M.Q., McCahon, D., Newman, J.W.I., Crowther, J.R., Carpenter, W.C. (1983). Electrofocusing Structural and Induced Proteins of Aphthovirus. In: Bachmann, P.A. (eds) New Developments in Diagnostic Virology. Current Topics in Microbiology and Immunology, vol 104. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68949-9_13
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DOI: https://doi.org/10.1007/978-3-642-68949-9_13
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