Mechanisms for Induction of Differentiation in the Human Promyelocytic Cell Line HL-60

  • I. L. Olsson
  • T. R. Breitman
  • M. G. Sarngadharan
  • R. C. Gallo
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 28)


Acute myeloid leukemia (AML) is characterized by a block in cell differentiation. Leukemic cell lines which grow continuously in vitro can be used to explore regulation of differentiation in leukemia. The promyelocytic HL-60 line [3] is induced to mature into granulocytes by incubation with agents such as dimethylsulfoxide (DMSO) [4] and retinoic acid (RA) [2]. The mechanisms by which these and other agents induce differentiation are unknown. Knowledge of them could improve understanding of the defects in terminal differentiation characteristic of AML. We have concentrated on mechanisms of action of RA, DIF (see below), and cAMP-inducing agents [1] because these act at low concentrations and most of them are regarded as physiological. Nitroblue tetrazolium (NBT) reduction has been used as the parameter of induced differentiation as it is a reliable measure of functional maturation in HL-60.


Acute Myeloid Leukemia Retinoic Acid Leukemic Cell Line Human Promyelocytic Leukemic Cell Line Human Myeloid Leukemic Cell Line 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • I. L. Olsson
  • T. R. Breitman
  • M. G. Sarngadharan
  • R. C. Gallo

There are no affiliations available

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