Mechanisms for Induction of Differentiation in the Human Promyelocytic Cell Line HL-60
Acute myeloid leukemia (AML) is characterized by a block in cell differentiation. Leukemic cell lines which grow continuously in vitro can be used to explore regulation of differentiation in leukemia. The promyelocytic HL-60 line  is induced to mature into granulocytes by incubation with agents such as dimethylsulfoxide (DMSO)  and retinoic acid (RA) . The mechanisms by which these and other agents induce differentiation are unknown. Knowledge of them could improve understanding of the defects in terminal differentiation characteristic of AML. We have concentrated on mechanisms of action of RA, DIF (see below), and cAMP-inducing agents  because these act at low concentrations and most of them are regarded as physiological. Nitroblue tetrazolium (NBT) reduction has been used as the parameter of induced differentiation as it is a reliable measure of functional maturation in HL-60.
KeywordsDMSO Leukemia Electrophoresis Polypeptide Polyacrylamide
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