Detection and Localization of a Phosphotyrosine-Containing onc Gene Product in Feline Tumor Cells

  • A. P. Chen
  • M. Essex
  • F. de Noronha
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 28)


Protein phosphorylation by a tyrosine-specific kinase is now recognized as a common event in retrovirus-transformed cells. We report in this communication that the feline sarcoma virus (FeSV) encoded transformation-specific proteins (gag-fes fusion proteins) and their associated protein kinases are also found in the FeSV in vivo induced tumor preparations, either in the form of fresh tumor homogenate or in the form of cultured cells. With the combined use of subcellular fractionation and detergent extraction we found that the protein kinase activity was present in both the membrane fraction (P100) and the cytosol (S100). The gag-fes proteins of two different strains of FeSV were found to associate with the cell framework to different degrees, suggesting that the specific conformational presentation of these proteins may be dictated by the unique portion of each polyprotein. The same gag-fes transformation related proteins could be immunoprecipitated with antiserum to phosphotyrosine.


Protein Kinase Activity Rous Sarcoma Virus Polyoma Virus Phosphoamino Acid Avian Sarcoma Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Barbacid M, Lauver AV, Devare SG (1980a) Biochemical and immunological characterizatioin of polyproteins coded for by the McDonough, Gardner-Arnstein, and Snyder-Theilen strains of feline sarcoma virus. J Virol 33: 196–207Google Scholar
  2. 2.
    Barbacid M, Beemon K, Devare SG (1980b) Origin and functional properties of the major gene product of the Snyder-Theilen strain of feline sarcoma virus. Proc Natl Acad Sci USA 77:5158–5163CrossRefGoogle Scholar
  3. 3.
    Beemon K, Ryden T, McNelly EA (1982) Transformation by avian sarcoma viruses leads to phosphorylation of multiple cellular proteins on tyrosine residues. J Virol 42:742–747PubMedGoogle Scholar
  4. 4.
    Bishop JM (1982) Oncogenes. Sci Amer 246:80–92Google Scholar
  5. 5.
    Blomberg J, Van de Ven WJM, Reynolds FH Jr, Nalewaik RP, Stephenson JR (1981) Snyder-Theilen feline sarcoma virus P85 contains a single phosphotyrosine acceptor site recognized by its associated protein kinase. J Virol 38: 886–894PubMedGoogle Scholar
  6. 6.
    Brown S, Levinson W, Spudick JA (1976) Cytoskeletal elements of chick embryo fibroblasts revealed by detergent extraction. J Supramol Struct 5: 119–130PubMedCrossRefGoogle Scholar
  7. 7.
    Chen AP, Essex M, Shadduck JA, Niederkorn JY, Albert D (1981) Retrovirus-encoded transformation-specific polyproteins: Expression coordinated with malignant phenotype in cells from different germ layers. Proc Natl Acad Sci USA 78:3915–3919PubMedCrossRefGoogle Scholar
  8. 8.
    Chen AP, Essex M, Kelliher M, deNoronha F, Shadduck JA, Niederkorn JY, Albert D (1983) Feline sarcoma virus-specific transformation related proteins and protein kinase activity in tumor cells. Virol 124:274–285CrossRefGoogle Scholar
  9. 9.
    Coffin J, Varmus HE, Bishop JM, Essex M, Hardy WD Jr, Martin S, Rosenberg NE, Scolnick EM, Weinberg RA, Vogt PK (1981) Proposal for naming host cell-derived inserts in retrovirus genomes. J Virol 40: 953–957PubMedGoogle Scholar
  10. 10.
    Collett M and Erikson RL (1978) Protein kinase activity associated with avian sarcoma virus src gene product. Proc Natl Acad Sci USA 75:2020–2024CrossRefGoogle Scholar
  11. 11.
    Cooper JA and Hunter T (1981) Changes in proein phosphorylation in Rous sarcom virus transformed chicken embryo cells. Molecular and Cellular Biology 1: 165–178PubMedGoogle Scholar
  12. 12.
    Courtneidge SA, Levison AD, Bishop JM (1980) The protein encoded by the transforming gene of avian sarcoma virus (pp60src) and a homologous protein in normal cells (pp60proto-src) are associated with the plasma membrane. Proc Natl Acad Sci USA 77:3783–3787PubMedCrossRefGoogle Scholar
  13. 13.
    Franchini G, Even J, Sherr CJ, Wong-Staal F (1981) Onc sequences (v-fes) of Snyder-Theilen feline sarcoma virus are derived from noncontiguous regions of a cat cellular gene (c-fes). Nature 290: 154–157PubMedCrossRefGoogle Scholar
  14. 14.
    Frankel AE, Gilbert JH, Porzig KJ, Scolnick EM, Aarsonson SA (1979) Nature and distribution of feline sarcom virus nucleotide sequences. J Virol 30: 821–827PubMedGoogle Scholar
  15. 15.
    Hardy WD Jr (1980) The biology and virology of the feline sarcoma virus. In: Hardy WD Jr, Essex M, McClelland J (eds) Feline leukemia and sarcoma viruses. Elsevier, New York pp 79–118Google Scholar
  16. 16.
    Hay AJ (1974) Studies on the formation of the influenza virus envelope. Virol 60:398–418CrossRefGoogle Scholar
  17. 17.
    Hunter T and Sefton BM (1980) Transforming gene product of Rous sarcoma virus phosphorylates tyrosiine. Proc Natl Acad Sci USA 77: 1311–1315PubMedCrossRefGoogle Scholar
  18. 18.
    Martinez R, Nakamura KD, Weber MJ (1982) Identification of phosphotyrosine-containing proteins in untransformed and Rous sarcoma virus-transformed chicken embryo fibroblasts. Mol Cell Biol 2:653–665PubMedGoogle Scholar
  19. 19.
    McCullough B, Shaller J, Shadduck JA, Yohn DS (1972) Induction of malignant melanoma associated with fibrosaromas in genotobiotic cats inoculated with Gardner-feline fibrobsarcoma virus. J Natl Cancer Inst 48: 1893–1896PubMedGoogle Scholar
  20. 20.
    Reynolds FH Jr, Van de Ven WJM, Stephenson JR (1980) Abelson murine leukemia virus transforming-defective mutants with impaired P120-asociated protein kinase activity. J Virol 36: 375–386Google Scholar
  21. 21.
    Rosenberg Z, and Haseltine W (1980) A transfection assay for transformation by feline sarcoma virus proviral DNA. Virol 102:240–244CrossRefGoogle Scholar
  22. 22.
    Ross AH, Baltimore D, Eisen H (1981) Phosphotyrosne-containingg proteins isolated by affinity chromatography with antibodies to a synthetic hapten. Nature 294:654–656PubMedCrossRefGoogle Scholar
  23. 23.
    Ruscetti SK, Turek LK, Sherr CJ (1980) Three independent isolates of feline sarcoma virus code for three distinct gag-x polyproteins. J Virol 35:259–264PubMedGoogle Scholar
  24. 24.
    Schaffhausen B and Benjamin TL (1979) Phosphorylation of polyoma T antigen. Cell 18:935–946PubMedCrossRefGoogle Scholar
  25. 25.
    Schaffhausen B, Benjamin TL (1981) Protein kinase activity associated with polyoma virus middle T antigen. Cold Spring Harbor Conf on Cell Prolif 8: 1281–1298Google Scholar
  26. 26.
    Sherr CJ, Fedele LA, Oskarsson M, Maizel J, Vande Woude G (1980) Molecular cloning of Snyder-Theilen feline leukemine and sarcoma viruses: Comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus. J Virol 34:200–212PubMedGoogle Scholar
  27. 27.
    Sefton BM, Hunter T, Beemon K, Eckhart W (1980) Evidence that the phosphorylation of tyrosine is essential for cellular transformation by Rous sarcoma virus. Cell 20:806–816CrossRefGoogle Scholar
  28. 28.
    Snyder H (1982) Biochemical characterization of protein kinase activities associated with transforming gene produts of the Snyder-Theilen and Gardner-Arnstein strain of feline sarcoma virus. Virol 117: 165–172CrossRefGoogle Scholar
  29. 29.
    Stephenson JR, Khan AS, Sliski AH, Essex M (1977) Feline oncornavirus-associated cell membrane antigen: Evidence for immunologically cross-reactive feline sarcoma virus coded protein. Proc Natl Acad Sci USA 75:5608–5612CrossRefGoogle Scholar
  30. 30.
    Ushiro H and Cohen S (1980) Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes. J Biol Chem 255: 8363–8365PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • A. P. Chen
  • M. Essex
  • F. de Noronha

There are no affiliations available

Personalised recommendations