Detection and Localization of a Phosphotyrosine-Containing onc Gene Product in Feline Tumor Cells

  • A. P. Chen
  • M. Essex
  • F. de Noronha
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 28)

Abstract

Protein phosphorylation by a tyrosine-specific kinase is now recognized as a common event in retrovirus-transformed cells. We report in this communication that the feline sarcoma virus (FeSV) encoded transformation-specific proteins (gag-fes fusion proteins) and their associated protein kinases are also found in the FeSV in vivo induced tumor preparations, either in the form of fresh tumor homogenate or in the form of cultured cells. With the combined use of subcellular fractionation and detergent extraction we found that the protein kinase activity was present in both the membrane fraction (P100) and the cytosol (S100). The gag-fes proteins of two different strains of FeSV were found to associate with the cell framework to different degrees, suggesting that the specific conformational presentation of these proteins may be dictated by the unique portion of each polyprotein. The same gag-fes transformation related proteins could be immunoprecipitated with antiserum to phosphotyrosine.

Keywords

Tyrosine Leukemia Manganese Fractionation Sarcoma 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • A. P. Chen
  • M. Essex
  • F. de Noronha

There are no affiliations available

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