Detection and Localization of a Phosphotyrosine-Containing onc Gene Product in Feline Tumor Cells
Protein phosphorylation by a tyrosine-specific kinase is now recognized as a common event in retrovirus-transformed cells. We report in this communication that the feline sarcoma virus (FeSV) encoded transformation-specific proteins (gag-fes fusion proteins) and their associated protein kinases are also found in the FeSV in vivo induced tumor preparations, either in the form of fresh tumor homogenate or in the form of cultured cells. With the combined use of subcellular fractionation and detergent extraction we found that the protein kinase activity was present in both the membrane fraction (P100) and the cytosol (S100). The gag-fes proteins of two different strains of FeSV were found to associate with the cell framework to different degrees, suggesting that the specific conformational presentation of these proteins may be dictated by the unique portion of each polyprotein. The same gag-fes transformation related proteins could be immunoprecipitated with antiserum to phosphotyrosine.
KeywordsProtein Kinase Activity Rous Sarcoma Virus Polyoma Virus Phosphoamino Acid Avian Sarcoma Virus
Unable to display preview. Download preview PDF.
- 1.Barbacid M, Lauver AV, Devare SG (1980a) Biochemical and immunological characterizatioin of polyproteins coded for by the McDonough, Gardner-Arnstein, and Snyder-Theilen strains of feline sarcoma virus. J Virol 33: 196–207Google Scholar
- 4.Bishop JM (1982) Oncogenes. Sci Amer 246:80–92Google Scholar
- 15.Hardy WD Jr (1980) The biology and virology of the feline sarcoma virus. In: Hardy WD Jr, Essex M, McClelland J (eds) Feline leukemia and sarcoma viruses. Elsevier, New York pp 79–118Google Scholar
- 20.Reynolds FH Jr, Van de Ven WJM, Stephenson JR (1980) Abelson murine leukemia virus transforming-defective mutants with impaired P120-asociated protein kinase activity. J Virol 36: 375–386Google Scholar
- 25.Schaffhausen B, Benjamin TL (1981) Protein kinase activity associated with polyoma virus middle T antigen. Cold Spring Harbor Conf on Cell Prolif 8: 1281–1298Google Scholar