Abstract
Mechanical stimulation of the skin surface in the presence of buffers containing Triton X-100 leads to the release of large numbers of corneocytes. These cells are essentially intact as judged by electron microscopy [1]. Even the corneocyte envelope appears unaltered by this treatment, suggesting that only intercellular and cell surface proteins are being released into the buffer. This material may be of physiological importance in desquamation, and in disease states such as ichthyosis. Here we describe the preparation and characterisation of non-ionic detergent soluble (NIDS) protein from normal human stratum corneum in vivo.
Keywords
- Stratum Corneum
- Phenylmethyl Sulphonyl Fluoride
- Chlorhexidine Gluconate
- Human Stratum Corneum
- Sulphonyl Fluoride
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
See end of contribution for alphabetization of abbreviations
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References
Barton SP, King CS, Marks R, Nicholls S (1980) A technique for studying the structural detail of isolated human corneocytes. Br J Dermatol 102: 63–74
Bjerrum OJ, Bog-Hansen TC (1976) Immunochemical gel precipitation techniques. In: Maddy AH (ed) Biochemical analysis of membranes. Chapman & Hall, London, pp 378–426
Hayward AF, Hackman M (1973) Electron microscopy of membrane-coating granules and a cell surface coat in keratinised and non keratinised human oral epithelium. J Ultrastruct Res 43: 205–219
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© 1983 Springer-Verlag Berlin Heidelberg
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King, C.S., Dykes, P.J., Marks, R. (1983). Preparation and Immunochemical Analysis of Non-Ionic Detergent-Soluble Protein From Human Stratum Corneum. In: Marks, R., Plewig, G. (eds) Stratum Corneum. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68682-5_8
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DOI: https://doi.org/10.1007/978-3-642-68682-5_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-11704-9
Online ISBN: 978-3-642-68682-5
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