Control of Involucrin Synthesis During Terminal Differentiation of Human Epidermal Cells in Culture

  • F. M. Watt


In the epidermis, cell division takes place in the basal layer. When cells leave this layer they no longer proliferate, but undergo terminal differentiation. Moving through the stratified layers, the cells enlarge progressively and synthesise a range of specialised products. In the outermost cell layers destruction of the nucleus and cytoplasmic organelles occurs, leaving only keratin fibres surrounded by a cross-linked protein envelope.


Epidermal Cell Stratum Corneum Basal Layer Terminal Differentiation Cornified Envelope 
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  1. 1.
    Banks-Schlegel SP, Green H (1980) Studies on the development of the definitive cell type of embryonic epidermis using the cross-linked envelope as a differentiation marker. Dev Biol 74: 275–285PubMedCrossRefGoogle Scholar
  2. 2.
    Banks-Schlegel S, Green H (1980) Formation of epidermis by serially cultivated human epidermal cells transplanted as an epithelium to athymic mice. Transplantation 29: 308–313PubMedCrossRefGoogle Scholar
  3. 3.
    Banks-Schlegel S, Green H (1981) Involucrin synthesis and tissue assembly by keratinocytes in natural and cultured human epithelia. J Cell Biol 90: 732–737PubMedCrossRefGoogle Scholar
  4. 4.
    Doran TI, Vidrich A, Sun T-T (1980) Intrinsic and extrinsic regulation of the differentiation of skin, corneal and esophageal epithelial cells. Cell 22: 17—25PubMedCrossRefGoogle Scholar
  5. 5.
    Fuchs E, Green H (1978) The expression of keratin genes in epidermis and cultured epidermal cells. Cell 15: 887–897PubMedCrossRefGoogle Scholar
  6. 6.
    Green H (1978) Cyclic AMP in relation to proliferation of the epidermal cell: a new view. Cell 15: 801–811PubMedCrossRefGoogle Scholar
  7. 7.
    Green H (1980) The keratinocyte as differentiated cell type. Harvey Lect 74: 101—139PubMedGoogle Scholar
  8. 8.
    Hennings H, Michael D, Cheng C, Steinert P, Holbrook K, Yuspa SH (1980) Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell 19: 245–254PubMedCrossRefGoogle Scholar
  9. 9.
    Moscona A (1961) Rotation-mediated histogenic aggregation of dissociated cells. A quantifiable approach to cell interations in vitro. Exp Cell Res 22: 455—475PubMedCrossRefGoogle Scholar
  10. 10.
    O’Connor NE, Mulliken JB, Banks-Schlegel S, Kehinde O, Green H (1981) Grafting of burns with cultured epithelium prepared from autologous epidermal cells. Lancet 1: 75–78CrossRefGoogle Scholar
  11. 11.
    Rheinwald JG, Green H (1975) Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6: 331–344PubMedCrossRefGoogle Scholar
  12. 12.
    Rheinwald JG, Green H (1977) Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature 265: 421–424PubMedCrossRefGoogle Scholar
  13. 13.
    Rice RH, Green H (1977) The cornified envelope of terminally differentiated human epidermal keratinocytes consists of cross-linked protein. Cell 11: 417–422PubMedCrossRefGoogle Scholar
  14. 14.
    Rice RH, Green H (1978) Relation of protein synthesis and transglutaminase activity to formation of the cross-linked envelope during terminal differentiation of the cultured human epidermal keratinocyte. J Cell Biol 76: 705–711PubMedCrossRefGoogle Scholar
  15. 15.
    Rice RH, Green H (1979) Presence in human epidermal cells of a soluble protein precursor of the cross-linked envelope: activation of the cross-linking by calcium ions. Cell 18: 681—694PubMedCrossRefGoogle Scholar
  16. 16.
    Sun T-T, Green H (1976) Differentiation of the epidermal keratinocyte in cell culture: formation of the cornified envelope. Cell 9: 511—521PubMedCrossRefGoogle Scholar
  17. 17.
    Sun T-T, Green H (1977) Cultured epithelial cells of cornea, conjunctiva and skin: absence of marked intrinsic divergence of their differentiated states. Nature 269: 489—493PubMedCrossRefGoogle Scholar
  18. 18.
    Sun T-T, Green H (1978) Immunofluorescent staining of keratin fibers in cultured cells. Cell 14: 469–476PubMedCrossRefGoogle Scholar
  19. 19.
    Watt FM, Green H (1981) Involucrin synthesis is correlated with cell size in human epidermal cultures. J Cell Biol 90: 738—742PubMedCrossRefGoogle Scholar
  20. 20.
    Watt FM, Green H (1982) Stratification and terminal differentiation of cultured epidermal cells. Nature 295: 434—436PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • F. M. Watt
    • 1
  1. 1.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA

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