Different Factors Active in Lymphoid and Hematopoietic Proliferation Produced by Single Clones of Helper T Cell Hybridomas

  • C. Corbel
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 100)


Single clones of antigen-specific, H-2-restrieted helper T cells have been found to produce a variety of lymphokines and hematopoietic factors when stimulated by antigen and adherent cells. These include factors which are active on lymphoid cells, i.e., T cell growth factor (TCGF) (1), B cell growth and maturation factor (BRMF) (2), and T cell replacing factor (TRF) (3). Various colony-stimulating activities (CSF) have been shown to stimulate proliferation of hematopoietic precursor cells. Such activities have also been described previously in supernatants from spleen cells stimulated with either mitogen (4,5) or antigen (6) or from the WEHI-3 macrophage cell line (7,8). The hybridoma technique can immortalize this capacity of normal T cells (9). T cell hybridomas offer advantages as a defined source of material for biochemical and genetic analysis of these different growth factors. It would, however, seem advantageous to have T cell hybridomas which produce only a single factor in order to determine their structure and to distinguish their activity with normal populations of cells from other activities with the same cell populations. I have therefore studied T cell hybridomas with different specificities from different fusion events as well as a series of reclones of a single T cell hybridoma. Of BRMF activities only the LPS blast-replicating activity has been determined, called B cell growth factor (BCGF) in the following. While the relative and absolute amounts of TCGF and BCGF vary even between different reclones of the same cloned T cell hybridoma I have not yet found a hybridoma producing only one activity.


Fetal Liver Cell CTLL Cell Hematopoietic Factor Soybean Lipid Erythroid Colony Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Gillis, S, Smith, KA (1977) Long-term culture of tumor-specific cytotoxic T cells. Nature 268: 154–156PubMedCrossRefGoogle Scholar
  2. 2.
    Melchers, F, Andersson, J, Lernhardt, W, Schreier, M (1980) H-2- unrestricted, polyclonal maturation without replication of small B cells induced by antigen-activated T cell help factors. Eur J Immunol 10: 679–685PubMedCrossRefGoogle Scholar
  3. 3.
    Schimpl, A, Wecker, E (1975) A third signal in B cell activation given by TRF. Transplant Rev 23: 176–188PubMedGoogle Scholar
  4. 4.
    Iscove, NN, Guilbert, LJ (1978) Erythropoietin-independence of early erythropoiesis and a two-regulator model of proliferative control in the hemopoietic system. In: Murphy Jr, MJ (ed) In Vitro Aspects of Erythropoiesis. Springer Verlag, New York, pp 3–7Google Scholar
  5. 5.
    Johnson, GR, Metealf, D (1977) Pure and mixed erythroid colony formation in vitro stimulated by spleen-conditioned medium with no detectable erythropoietin. Proc Natl Acad Sci USA 74: 3879–3882PubMedCrossRefGoogle Scholar
  6. 6.
    Schreier, MH, Iscove, NN (1980) Haematopoietic growth factors are released in cultures of H-2-restricted helper T cells, accessory cells and specific antigen. Nature 287: 228–230PubMedCrossRefGoogle Scholar
  7. 7.
    Iscove, NN, Schreier, MH (1979) Involvement of T cells and macrophages in generation of burst-promoting activity. Exp Hemat 7: 4.Google Scholar
  8. 8.
    Wagemaker, G (1980) Early erthropoietin-independent stage of in vitro erythropoiesis: Relevance to stem cell differentiation. In: Braun, SJ, Ledney, GD, van Bekkum, DW (eds) Experimental Hematology Today, 1980. Karger, Basel, pp 47–60Google Scholar
  9. 9.
    Schrader, JW, Arnold, B, Clark-Lewis, I (1980) A Con A-stimulated T-cell hybridoma releases factors affecting haematopoietic colony-forming cells and B cell antibody responses. Nature 283: 197–199PubMedCrossRefGoogle Scholar
  10. 10.
    Melchers, F, Zeuthen, J, Gerhard, W (1982) Influenza virus- specific murine T cell hybridgmas which recognize virus hemagglutinin in conjunction with H-2 and display helper functions for B cells. (This volume.)Google Scholar
  11. 11.
    Hartmann, K-U (1970) Induction of a hemolysin response in vitro. Interaction of cells of bone-marrow origin and thymic origin. J Exp Med 132: 1267–1278PubMedCrossRefGoogle Scholar
  12. 12.
    Iscove, NN, Melchers, F (1978) Complete replacement of serum by albumin, transferrin and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med 147: 923–933PubMedCrossRefGoogle Scholar
  13. 13.
    Gillis, S, Ferm, MM, Ou, W, Smith, KA (1978) T cell growth factors: Parameters of production and a quantitative microassay for activity. J Immunol 120: 2027–2032PubMedGoogle Scholar
  14. 14.
    Miller, RG, Phillips, RA (1969) Separation of cells by velocity sedimentation. J Cell Physiol 73: 191–201PubMedCrossRefGoogle Scholar
  15. 15.
    Andersson, J, Schreier, MH, Melchers, F (1980) T-cell-dependent B-cell stimulation is H-2-restricted and antigen-dependent only at the resting B-cell level. Proc Natl Acad Sci USA 77: 1612–1616PubMedCrossRefGoogle Scholar
  16. 16.
    Trowbridge, IS, Hyman, R, Mazauskas, C (1978) The synthesis and properties of T9t. glycoprotein in Thy-l-negative mutant lymphoma cells. Cell 14: 21–32PubMedCrossRefGoogle Scholar
  17. 17.
    Gronvik, K-O, Andersson, J, Melchers, F, Zeuthen, J (1982) A stable TCGF-producing T cell hybridoma and its thioguanine-resistant variant suitable as a tool for the construction of new functional T hybridomas. (This volume.)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • C. Corbel

There are no affiliations available

Personalised recommendations