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Production of Antigen-Nonspecific Immunoregulatory Lymphokines by T Cell Hybridomas

  • A. Altman
  • R. D. Schreiber
  • D. H. Katz
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 100)

Abstract

A large number of non-immunoglobulin soluble mediators or lymphokines (LK) with immunoregulatory and other biological activities have been studied over the last 12 years. The majority of these LK are products of T cells activated by antigens or polyclonal mitogens. Studies on T cell-derived LK have been severely hampered by the limited amounts of LK available from conventional cultures and the heterogeneity of the cell populations producing these LK which, in turn, resulted in heterogeneous mixtures of many distinct LK. As a result of these limitations, it has been difficult to delineate the exact chemical nature of LK, the structure-function relationship among various biological activities and their biological significance.

Keywords

Migration Inhibitory Factor Macrophage Activate Factor Cell Stimulate Activity Macrophage Tumoricidal Activity Costimulator Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Tada T, Okumura K (1979) The role of antigen—specif ic cell factors in the immune response. Adv Immunol 28: 1–87PubMedCrossRefGoogle Scholar
  2. 2.
    Eshhar Z (to be published) Functional T cell hybridomas producing antigen-specific immunoregulatory factors. In: Katz DH (ed) Monoclonal antibodies and T cell products. CRC Press, Inc., Boca Raton, FLGoogle Scholar
  3. 3.
    Altman A, Katz DH (to be published) The biology of monoclonal lymphokines secreted by T cell lines and hybridomas. Adv ImmunolGoogle Scholar
  4. 4.
    Armerding D, Katz DH (1974) Activation of T and B lymphocytes in vitro. II. Biological and biochemical properties of an allogeneic effect factor (AEF) active in triggering specific B lymphocytes. 3 Exp Ned 140: 19–37Google Scholar
  5. 5.
    Armerding D, Sachs DH, Katz DH (1974) Activation of T and B lymphocytes in vitro. III. Presence of Ia determinants on allogeneic effect factor (AEF). 3 Exp Med 140: 1717–1722CrossRefGoogle Scholar
  6. 6.
    Eshhar Z, Armerding D, Waks T, Katz DH (1977) Activation of T and B lymphocytes in vitro. V. Cellular locus, metabolism and genetics of induction and production of the allogeneic effect factor. 3 Immunol 119: 1457–1467Google Scholar
  7. 7.
    Armerding D, Eshhar Z, Katz DH (1977) Activation of T and B lymphocytes in vitro. VI. Bochemical and physicochemical characterization of the allogeneic effect factor. 3 Immunol 119: 1468–1474Google Scholar
  8. 8.
    Altman A, Katz DH (1980) Stimulation of autoreactive T lymphocytes by allogeneic effect factor (AEF): Relevance to normal pathways of lymphocyte differentiation. Immunol Rev 51: 3–34PubMedCrossRefGoogle Scholar
  9. 9.
    Altman A, Katz DH (1980) Existence of T cells manifesting self-reactivity indistinguishable from alloreactivity. 3 Immunol 125: 1536–1543Google Scholar
  10. 10.
    Watson R, Mochizuki D, Gillis S (1980) T cell growth factors: interleukin 2. Immunol Today 1: 113–117CrossRefGoogle Scholar
  11. 11.
    Smith KA, Ruscetti FW (to be published) T cell growth factor and the culture of cloned functional T cells. Adv ImmunolGoogle Scholar
  12. 12.
    Altman A, Gilmartin TD, Katz DH (1981) Bone marrow stem cell differentiation in vitro: Promotion of long term growth by a soluble lymphocyte-derived mediator. Science 211: 65–67, 1981PubMedCrossRefGoogle Scholar
  13. 13.
    Altman A, Cardenas JM, Welsh RM, Jr, Katz DH (1980) The biological effects of allogeneic effect factor on T lymphocytes. III. Interferon does not contribute to the biological activities displayed by AEF on both T and B lymphocytes. Ann Immunol (Inst Pasteur) 131C: 335–347Google Scholar
  14. 14.
    Katz DH, Bechtold TE, Altman A (1980) Construction of T cell hybridomas secreting allogeneic effect factor (AEF). 3 Exp Med 152: 956–968Google Scholar
  15. 15.
    Altman A, Schreiber RD, Katz DH (to be published) Functional T cell hybridomas producing non-specific Immunoregulatory factors. I n: Katz DH (ed) Monoclonal antibodies and T cell products. CRC Press, Inc., Boca Raton, FLGoogle Scholar
  16. 16.
    Altman A, Sferruzza A, Weiner RG, Katz DH (to be published) Constitutive and mitogen-induced production of T cell growth factor by stable T cell hybridoma lines. 3 ImmunolGoogle Scholar
  17. 17.
    Schreiber RD, Altman A, Katz DH (submitted for publication) Identificati on of a T cell hybridoma which produces extraordinary quantities of macrophage activating factorGoogle Scholar
  18. 18.
    Gefter ML, Margulies DH, Scharff MD (1977) Simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells. Somatic Cell Genet 3: 231–236PubMedCrossRefGoogle Scholar
  19. 19.
    Lake P, Clark EA, Khorshidi M, Sunshine GH (1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets. Eur 3 Immunol 9: 875–886CrossRefGoogle Scholar
  20. 20.
    Gillis S, Smith KA (1977) Long term culture of tumour-specific cytotoxic T cells. Nature (London) 268: 154–156CrossRefGoogle Scholar
  21. 21.
    Gillis S, Ferm MM, Ou W, Smith KA (1978) T cell growth factor: Parameters of production and a quantitative microassay for activity. 3 Immunol 120: 2027–2032Google Scholar
  22. 22.
    Paetkau V, Mills G, Gerhart G, Monticone V (1976) Proliferation of murine thymic lymphocytes in vitro mediated by the concanavalin A-induced release of a lymphokine (costimulator). 3 Immunol 117: 1320–1324Google Scholar
  23. 23.
    Altman A, Cardenas JM, Bechtold TE, Katz DH (1980) The biological effects of allogeneic effect factor on T lymphocytes. I. The mitogenic activity and the autonomous induction of cytotoxic T lymphocytes by AEF. 3 Immunol 124: 105–113Google Scholar
  24. 24.
    Swain SL, Dennert G, Warner JF, Dutton RW (1981) Culture supernatants of a stimulated T cell line have helper activity that synergizes with IL-2 in the response of B cells to antigen. Proc Natl Acad Sci USA 78: 2517–2521PubMedCrossRefGoogle Scholar
  25. 25.
    Takatsu K, Tanaka K, Tominaga A, Kumahara Y, Hamaoka T (1980) Antigen-induced T cell-replacing factor (TRF). III. Establishment of T cell hybrid clone continuously producing TRF and functional analysis of released TRF. 3 Immunol 125: 2646–2653Google Scholar
  26. 26.
    Pure E, Isakson PC, Takatsu K, Hamaoka T, Swain SL, Dutton RW, Dennert G, Uhr RW, Vitetta ES (1981) Induction of B cell differentiation by T cell factors. I. Stimulation of IgM secretion by products of a T cell hybridoma and a T cell line. 3 Immunol 127: 1953–1958Google Scholar
  27. 27.
    Howard M, Kessler S, Chused T, Paul WE (1981) Long-term culture of normal mouse B lymphocytes. Proc Natl Acad Sci USA 78: 5788–5792PubMedCrossRefGoogle Scholar
  28. 28.
    Watson J, Gillis S, Marbrook R, Mochizuki D, Smith KA (1979) Biochemical and biological characterization of lymphocyte regulatory molecules. I. Purification of a class of murine lymphokines. 3 Exp Med 150: 849–861CrossRefGoogle Scholar
  29. 29.
    Kniep EM, Domzig W, Lohmann-Matthes M-L, Kickhofen B (1981) Partial purification and chemical characterization of macrophage cytotoxicity factor (MCF, MAF) and its separation from migration inhibitory factor (MIF). 3 Immunol 127: 417–422Google Scholar
  30. 30.
    Schultz RM, Chirigos MA (1978) Similarities among factors that render macrophages tumoricidal in lymphokine and interferon preparations. Cancer Res 38: 1003–1007PubMedGoogle Scholar
  31. 31.
    Jett J, Mantovani A, Herberman RB (1980) Augmentation of monocyte-mediated cytolysis by interferon. Cell Immunol 54: 425–434PubMedCrossRefGoogle Scholar
  32. 32.
    Youngner JS, Salvin SB (1973) Production and properties of migration inhibitory factor and interferon in the circulation of mice with delayed hypersensitivity. 3 Immunol 111: 1914–1922Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • A. Altman
  • R. D. Schreiber
  • D. H. Katz

There are no affiliations available

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