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Functional Characteristics of T Cell Hybridomas Obtained by Fusion of TCGF-Dependent Helper T Cell Clones with BW5147

  • M. H. Schreier
  • R. Tees
  • L. Nordin
  • A. A. Nordin
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 100)

Abstract

Over the last three years we have established a large panel of functionally active antigen-specific T cell clones with helper T cell characteristics (1–3). Such T cells are reasonably stable and have been propagated in vitro in TCGF-supplemented medium for up to two years. Their extensive characterization in vitro and in vivo has shown that the same cell can express multiple functions and has revealed a remarkable functional heterogeneity among individual clones (4,5). Since long-term propagation of these TCGF-dependent clones is demanding and involves an expensive and time-consuming cell culture technology (4,6), it was obvious to aim at a more convenient source of monoclonal T cells with comparable functional properties. We have therefore established a large panel of T cell hybridomas by fusing the HAT-sensitive thymoma cell line BW5147 with various TCGF-dependent specific helper clones which had been shown to release biologically active factors when interacting with I-A-compatible macrophages and antigen. This allowed us to answer two questions: Are functional properties of TCGF-dependent helper clones expressed in T cell hybridomas? And, is there any correlation between TCGF production and helper activity for the in vitro antibody response?

Keywords

Antibody Response Cell Clone Spleen Cell Cell Hybrid Helper Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • M. H. Schreier
  • R. Tees
  • L. Nordin
  • A. A. Nordin

There are no affiliations available

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