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Lectin-Dependent Cytolytic and Cytolymic T Helper Clones and Hybridomas

  • K. Fischer Lindahl
  • A. A. Nordin
  • M. H. Schreier
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 100)

Abstract

Any cytotoxic T lymphocyte (CTL) will kill P815 or EL4 target cells on addition of a lectin such as phytohemagglutinin (PHA) or concanavalin A (Con A) (1,2), and lectin-dependent killing has been considered a prerogative of CTL (3). However, lectin-dependent killers could be found under conditions where no specific CTL were induced (4–6), and nearly every T cell clone stimulated by Con A and T cell growth factor (TCGF) caused 51Cr-release in the presence of PHA (7). It therefore seemed possible that any T cell can become a lectin-dependent killer. To find out how common this activity might be, we tested a set of antigen-specific, I-A-restricted T helper cell lines, clones and hybridomas (8–10).

Keywords

Effector Cell Spleen Cell Helper Cell Spontaneous Release P81S Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Zagury, M, Fouchard, M, Morgan, DA, Cerottini, JC (1980) Enumeration of T-effector cells mediating direct and/or lectin-dependent lysis. Immunol Letters 1: 335–339CrossRefGoogle Scholar
  2. 2.
    Bradley, TP, Bonavida, B (1981) Mechanism of cell-mediated cytotoxicity at the single cell level. III. Evidence that cytotoxic T lymphocytes lyse both antigen-specific and -nonspecific targets pretreated with lectins or periodate. J Immunol 126: 208–213PubMedGoogle Scholar
  3. 3.
    Bevan MJ, Cohn, M (1975) Cytotoxic effects of antigen- and mitogen-induced T cells on various targets. J Immunol 114: 559–565PubMedGoogle Scholar
  4. 4.
    Rubens, RP, Henney, CS (1977) Studies on the mechanism of lymphocyte-mediated cytolysis. VIII. The use of Con A to delineate a distinctive killer T cell subpopulation. J Immunol 118: 180–186PubMedGoogle Scholar
  5. 5.
    Davignon, DJ, Laux, DC (1978) Lectin-dependent cell-mediated cytotoxicity: Induction of a unique effector cell population. Cell Immunol 41: 294–303PubMedCrossRefGoogle Scholar
  6. 6.
    Cerottini, JC, MacDonald, HR (1981) Limiting dilution analysis of alloantigen-reactive T lymphocytes. V. Lyt phenotype of cytolytic T lymphocyte precursors reactive against normal and mutant H-2 antigens. J Immunol 126: 490–496PubMedGoogle Scholar
  7. 7.
    Larsson, EL, Fischer Lindahl, K, Langhorne, J, Coutinho, A (1981) Quantitative studies on Concanavalin A-induced, TCGF-reactive T cells. 1. Correlation between proliferation and lectin-dependent cytolytic activity. J Immunol 127: 1081–1085PubMedGoogle Scholar
  8. 8.
    Schreier, MH, Tees, R (1980) Clonal induction of helper T cells: Conversion of specific signals into nonspecific signals. Int Archs Allergy Appl Immun 61: 227–237CrossRefGoogle Scholar
  9. 9.
    Schreier, MH, Tees, R, Nordin, AA (1981) Establishment and characterization of helper T cell clones. Their functional heterogeneity and effect on the B cell responses to particulate, soluble and T independent antigens. In: Feldmann, M, Schreier, MH (eds) Lymphokines 5: Monoclonal T Cells and Their Products. Academic Press, New York, in pressGoogle Scholar
  10. 10.
    Schreier, MH, Tees, R, Nordin, AA, Benner, R, Bianchi, ATJ, van Zwieten, MJ (1982) Functional aspects of helper T cell clones. In: Smith, KA (ed) The growth and functional aspects of T cells. Immunobiology Supplement, in pressGoogle Scholar
  11. 11.
    Schreier, MH, Tees, R (1982) Long-term culture and cloning of specific helper T cells. In: Pernis, B, Lefkovits, I (eds) Immunological Methods, Vol. II. Academic Press, New York, pp 263–275Google Scholar
  12. 12.
    Schreier, MH, Tees, R, Nordin, L, Nordin, AA (1982) Functional characteristics of T cell hybridomas obtained by fusion of TCGF-dependent helper T cell clones with BW5147. (This volume.)Google Scholar
  13. 13.
    Fischer Lindahl, K, Hausmann, B (1980) Qed-l — a target for unrestricted killing by T cells. Eur J Immunol 10: 289–298PubMedCrossRefGoogle Scholar
  14. 14.
    Fazekas de St.Groth, S, Scheidegger, D (1980) Production of monoclonal antibodies: Strategy and tactics. J Imm Meths 35: 1–21CrossRefGoogle Scholar
  15. 15.
    Fischer Lindahl, K, Schreier, MH (1981) Submitted for publication.Google Scholar
  16. 16.
    Lees, RK, MacDonald, HR, Sinclair, NR (1977) Inhibition of clone formation as an assay for T cell-mediated cytotoxicity: Short-term kinetics and comparison with 51Cr release. J Imm Meths 16: 233–244CrossRefGoogle Scholar
  17. 17.
    Dennert, G, Weiss, S, Warner, JF (1981) T cells may express multiple activities: specific allohelp, cytolysis, and delayed-type hypersensitivity are expressed by a cloned T-cell line. Proc Natl Acad Sci USA 78: 4540–4543PubMedCrossRefGoogle Scholar
  18. 18.
    Dialynas, DP, Loken, MR, Glasebrook, AL, Fitch, FW (1981) Lyt-2- /Lyt-3- variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis. J Exp Med 153: 595–604PubMedCrossRefGoogle Scholar
  19. 19.
    Lemke, H, Hämmerling, GJ, Höhmann, C, Rajewsky, K (1978) Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Nature 271: 249–251PubMedCrossRefGoogle Scholar
  20. 20.
    Köhler, G, Fischer Lindahl, K, Heusser, C (1981) Characterization of a monoclonal anti-H-2Kb antibody. In: Steinberg, CM, Lefkovits, I (eds) The Immune System, 2. S. Karger, Basel, pp 202–208Google Scholar
  21. 21.
    Marshak-Rothstein, A, Fink, P, Gridley, T, Raulet, DH, Bevan, MJ, Gefter, ML (1979) Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J Immunol 122: 2491–2497PubMedGoogle Scholar
  22. 22.
    Ledbetter, JA, Herzenberg, LA (1979) Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47: 63–90PubMedCrossRefGoogle Scholar
  23. 23.
    Gottlieb, PD, Marshak-Rothstein, A, Auditore-Hargreaves, K, Berkoben, DB, August, DA, Rosche, RM, Benedetto, JD (1980) Construction and properties of new Lyt-congenic strains and anti-Lyt-2.2 and anti-Lyt 3.1 monoclonal antibodies. Immunogenetics 10: 545–555CrossRefGoogle Scholar
  24. 24.
    Sarmiento, M, Glasebrook, AL, Fitch, FW (1980) IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement. J Immunol 125: 2665–2672PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1982

Authors and Affiliations

  • K. Fischer Lindahl
  • A. A. Nordin
  • M. H. Schreier

There are no affiliations available

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