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Influence of Light on Human Immunocompetent Cells in Vitro

  • H. Lederer
  • K. Stönkel
  • R. Denk
  • W. Waidelich
Conference paper

Abstract

The effect of monochromatic electromagnetic radiation in the visible spectral range on human peripheral leucocytes was investigated in allogeneic and mitogenic stimulations as well as in migration inhibition assays. For irradiation we used HeNe-, Ar+-, Kr+-lasers and a thermal light source. The influence of the following parameters of the applied light was considered: wavelength, power density, exposure time, state of polarization and particularly the coherence. No influence of irradiation with any light source was seen in mixed lymphocyte reactions and mitogenic stimulations. In contrast irradiation with coherent HeNe-laser light effected leucocytes in migration inhibition assays. Linearly polarized incoherent light of the same wavelength and power density showed no influence.

Keywords

Power Density Mixed Lymphocyte Reaction Mitogenic Stimulation Migration Area Incoherent Light 
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References

  1. 1.
    MESTER, E., JASZSAGI-NAGI, E, BACSY, E., KORENYI-GOTH, A., NEMENYI, K., KOVACS, I., TISZA, S.: Laser in Medizin — experimentelle und klinische Erfahrungen. In: Med. Physik in Forschung und Praxis 117–137. Berlin-New York: Walther de Gruyter 1976Google Scholar
  2. 2.
    HAINA, D., HUTSCHENREITER, G., SEIPP, W., HOHENFELLNER, R., WAIDELICH, W.: Versuche zur Wundheilung mit Laserstrahlung. In: Laser 77 Opto-Electronics, Conference Proceedings 501–507. IPC Science and Technology Press Ltd. 1977 (ed. by Waidelich, W.)Google Scholar
  3. 3.
    HUTSCHENREITER, G., HAINA, D., PAULIN, K., SCHUMACHER, G.: Wundheilung nach Laser- und Rotlichtbestrahlung. Z. exp. Chir. 13, 75–85 (1980)PubMedGoogle Scholar
  4. 4.
    KANA, J.S., HUTSCHENREITER, G., HAINA, D.: The Effect of Low Power Density Laser Radiation on the Healing of Open Skin Wounds in Rats. Arch. Surg. 116, 293–296 (1981)PubMedGoogle Scholar
  5. 5.
    MESTER, E., NAGYLUCSKAY, S., WAIDELICH, W., TISZA, S., GREGUSS, P., HAINA, D., MESTER, A.: Effects of Direct Laser Radiation on Human Lymphocytes. Arch. Dermatol. Res. 263, 241–245 (1978)PubMedCrossRefGoogle Scholar
  6. 6.
    BOYUM, A.: Separation of leucocytes from blood and bone marrow. Scan. J. Lab. Invest. 21 Suppl. 97 (1968)Google Scholar
  7. 7.
    OPPENHEIM, J.J., ROSENSTREICH, D.L.: Mitogens in Immunobiology. New York-San Francisco-London: Academic Press 1976Google Scholar
  8. 8.
    OPPENHEIM, J.J., ROSENSTREICH, D.L. in: In Vitro Methods of Cell-Mediated and Tumor Immunity 573 ff (ed. by Bloom B., David, J.)Google Scholar
  9. 9.
    WEESE, J.L., McCOY, J.L., DEAN, J.H., ORTALDO, J.R., BURK, K.R., HERBERMAN, R.B.: Brief Communication: Technical Modifications of the Human Agarose Microdroplet Leucocyte Migration Inhibition Assay. J. Immunol. Methods 24, 363–370 (1980)CrossRefGoogle Scholar
  10. 10.
    FROHLICH, H.: Private Communication (1981)Google Scholar

Copyright information

© Springer-Verlag Berlin, Heidelberg 1982

Authors and Affiliations

  • H. Lederer
    • 1
  • K. Stönkel
    • 1
    • 2
  • R. Denk
    • 1
  • W. Waidelich
    • 1
  1. 1.Institut für Medizinische OptikUniversität MünchenMünchen 40Germany
  2. 2.Hamatologisches InstitutGSFMünchen 2Germany

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