Abstract
An important prerequisite in the study of erythrocyte membrane structure and function is the availability of suitable membrane preparations. Several methods by which such “ghosts” can be prepared have been published by various authors. A summary of some of these methods has been presented by Schwoch and Passow (1973). Intact ghosts, containing all intrinsic (intramembraneous) and extrinsic proteins, as well as cytoskeletal components, can further be converted to yield membrane fragments such as spectrin-depleted ghost or inside-out vesicles, lacking one or more membrane constituents (Steck, 1974).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Lutz HU, Lio S-C, Palek J (1977) J Cell Biol 73:548–560
Ott P, Hope MJ, Verkleij AJ, Roelofsen B, Brodbeck U, van Deenen LLM (1981) Biochim Biophys Acta 641:79–87
Schwoch G, Passow H (1973) Mol Cell Biochem 2: 197–217
Steck TL (1974) Methods Membrane Biol 2: 245–281
van Deenen LLM, de Gier J (1974) In: Surgenor D (ed) The red blood cell, 2nd ed, vol 1. Academic Press, New York, pp 147–211
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1981 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Ott, P., Brodbeck, U. (1981). Preparation of Spectrin-Free Vesicles from Human Red Blood Cells. In: Azzi, A., Brodbeck, U., Zahler, P. (eds) Membrane Proteins. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68077-9_8
Download citation
DOI: https://doi.org/10.1007/978-3-642-68077-9_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-10749-1
Online ISBN: 978-3-642-68077-9
eBook Packages: Springer Book Archive