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Techniques in Neuroanatomical Research pp 21–40Cite as

General Methods in Transmission Electron Microscopy of the Nervous System

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Abstract

The application of electron microscopy to morphological, neuroanatomical research resulted in the development of more refined methods of obtaining reliable electron micrographs in an attempt to show the corresponding counterparts of living structures. In comparison to other organs, the preparation of specimens from the nervous system requires careful attention due to the particularities of the soft nervous tissue. The central nervous system is quite fragile with regard to mechanical stress and sensitive to lack of oxygen. Thus, the use of immersion fixation results in many artifacts which can be caused by mechanical damage and slow penetration of fixatives.

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Authors
  1. Wolf-Georg Forssmann
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Editors and Affiliations

  1. Anatomisches Institut der Universität, Im Neuenheimer Feld 307, 6900, Heidelberg, Federal Republic of Germany

    Christine Heym (I. Lehrstuhl) & Wolf-Georg Forssmann (III. Lehrstuhl) (I. Lehrstuhl) &  (III. Lehrstuhl)

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© 1981 Springer-Verlag Berlin Heidelberg

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Forssmann, WG. (1981). General Methods in Transmission Electron Microscopy of the Nervous System. In: Heym, C., Forssmann, WG. (eds) Techniques in Neuroanatomical Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68029-8_2

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  • DOI: https://doi.org/10.1007/978-3-642-68029-8_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-68031-1

  • Online ISBN: 978-3-642-68029-8

  • eBook Packages: Springer Book Archive

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