Abstract
The dynamic behavior of the extracellular ions of the brain has been revealed with a new clarity through the use of ion-selective micropipettes (ISMs) Numerous studies have revealed changes in [K+]0, [Ca2+]0, [Na+]0 and [Cl-]0 (see ref. 10 for review). One can go beyond the study of these endogenous brain ions, however, by the introduction of exogenous ionic “probes”, and the subsequent monitoring of their behavior with appropriately chosen ion exchangers in ISMs. In this paper we describe the use of anions, novel to the brain, as probes for the diffusion properties of the extracellular space, and as indicators of extracellular volume changes and changes in membrane permeability during spreading depression.
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References
Bures J, Buresova O, Krivanek J (1974) The mechanism and applications of Leao’s spreading depression of electroencephalographic activity. Academic Press, New York
Coetzee CJ, Freiser H (1969) Liquid–liquid membrane electrodes based on ion association extraction systems. Anal Chem 41: 1128–1130
Dietzel I, Heinemann U, Hofmeier G, Lux HD (1980) Transient changes in the size of the extracellular space in the sensorimotor cortex of cats in relation to stimulus induced changes in potassium concentrations. Exp Brain Res (in press)
Eisenman G (1968) Similarities and differences between liquid and solid ion exchangers and their usefulness as ion specific electrodes. Anal Chem 40: 310–320
Hansen AJ, Olsen CE (1980) Brain extracellular space during spreading depression and ischemia. Acta Physiol Scand 108: 355–365
Ishibashi N, Kohara H, Horinouchi K (1973) Aromatic sulfonate ion selective electrode membrane with Crystal Violet as ion exchange site. Talanta 20: 867
Kraig RP, Nicholson C (1978) Extracellular ionic variations during spreading depression. Neurosci 3: 1045–1059
Leao AAP (1944) Spreading depression of activity in the cerebral cortex. J Neurophysiol 7: 359–390
Lux HD (1974) Fast recording ion specific microelectrodes: Their use in pharmacological studies in the CNS. Neuropharmacol 13: 509–517
Nicholson C (1980) Dynamics of the brain cell microenvironment. Neurosci Res Prog Bull 18: 177–322
Nicholson C, ten Bruggencate G, Stockle H, Steinberg R (1978) Calcium and potassium changes in extracellular microenvironment of cat cerebellar cortex. J Neurophysiol 41: 1026–1039
Nicholson C, Kraig RP (1980) The behavior of extracellular ions during spreading depression. In: Zeuthen T (ed) The application of ion–selective electrodes. Elsevier/North–Holland, Amsterdam
Nicholson C, Phillips JM, Gardner-Medwin AR (1979) Diffusion from an ion-tophoretic point source in the brain: Role of tortuosity and volume fraction. Brain Res 169: 580–584
Nicholson C, Phillips JM (1979) Diffusion of anions and cations in the extracellular micro–environment of the brain. J Physiol 296: 66 P
Phillips JM, Nicholson C (1979) Anion permeability in spreading depression investigated with ion sensitive microelectrodes. Brain Res 173: 567–571
Schmitt FO, Samson FE Jr (1969) Brain cell microenvironment. Neurosci Res Prog Bull 7: 277–417
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© 1981 Springer-Verlag Berlin Heidelberg
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Phillips, J.M., Nicholson, C. (1981). Microelectrodes for Novel Anions and their Application to Some Neurophysiological Problems. In: Lübbers, D.W., Acker, H., Buck, R.P., Eisenman, G., Kessler, M.D., Simon, W. (eds) Progress in Enzyme and Ion-Selective Electrodes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67924-7_3
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DOI: https://doi.org/10.1007/978-3-642-67924-7_3
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-10499-5
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