Intracellular pH, Na+ and Ca2+ Activity Measurements in Mammalian Heart Muscle

  • D. Ellis
  • J. W. Deitmer
  • D. M. Beers


Ion-selective microelectrodes are now available for many ionic species so that studies are now feasible on the effects of changes in the activity of one type of ion on the activity of other ions in the cell. From initial studies on the control of intracellular Na+ activity (aNai) in cardiac tissues we became interested in transmembrane Na+–Ca2+ exchange. From our experiments we would have predicted that under some conditions a reduction of extracellular Na+, ([Na]o), would produce large charges in extracellular Ca2+ activity (aCai). These changes in aCai would however also probably change intracellular pH (pHi) as it has been shown that pressure injection of Ca2+ into snail neurones results in intracellular acidification (10). Therefore under conditions that produce large changes in internal Ca2+ the pHi would also be expected to alter. Changes in aCai could be demonstrated by the use of Ca2+-selective microelectrodes and by measuring the tension that the muscle developed.


Purkinje Fibre Intracellular Acidification Glass Microelectrode Snail Neurone Cardiac Purkinje Fibre 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bartley W. Amoore JE (1958) The effects of manganese on the solute content of rat liver mitochondria. Biochem J 69: 348PubMedGoogle Scholar
  2. 2.
    Deitmer JW, Ellis D (1978a) Changes in the intracellular sodium activity of sheep heart Purkinje fibres produced by calcium and other divalent cations. J Physiol 277: 437PubMedGoogle Scholar
  3. 3.
    Deitmer JW, Ellis D (1978b) The intracellular sodium activity of cardiac Purkinje fibres during inhibition and reactivation of the Na-K pump. J Physiol 284: 241PubMedGoogle Scholar
  4. 4.
    Deitmer JW, Ellis D (1980a) The intracellular sodium activity of sheep heart Purkinje fibres: Effects of local anaesthetic and tetrodotoxin. J Physiol 300: 269Google Scholar
  5. 5.
    Deitmer JW, Ellis D (1980b) Interactions between the regulation of the intracellular pH and sodium activity of sheep cardiac Purkinje fibres. J Physiol 304: 471PubMedGoogle Scholar
  6. 6.
    Ellis D (1977) The effect of external cations and ouabain on the sodium activity in sheep heart Purkinje fibres. J Physiol 273: 211PubMedGoogle Scholar
  7. 7.
    Ellis D, Thomas RC (1976) Direct measurement of the intracellular pH of mammalian cardiac tissue. J Physiol 262: 755PubMedGoogle Scholar
  8. 8.
    Fabiato A, Fabiato F (1978) Effects of pH on the myofilaments and the sarcoplasmic reticulum of skinned cells from cardiac and skeletal muscle. J Physiol 276: 233PubMedGoogle Scholar
  9. 9.
    Glitsch HG, Reuter H, Scholz H (1970) The effect of the internal sodium concentration on calcium fluxes in isolated guinea–pig auricles. J Physiol 209: 25PubMedGoogle Scholar
  10. 10.
    Meech RW, Thomas RC (1977) The effect of calcium injection on the intracellular sodium and pH of snail neurones.Google Scholar
  11. 11.
    Niedergerke R (11963 ) Movements of Ca in frog ventricles at rest and during contractures. J Physiol 167: 515Google Scholar
  12. 12.
    Reuter H, Seitz N (1968) The dependence of calcium efflux from cardiac muscle on temperature and external ion composition. J Physiol 195: 451PubMedGoogle Scholar
  13. 13.
    Oehme M, Kessler M, Simon W (1976) Neutral carrier Ca2+-microelectrode. Chimica 30: 204Google Scholar
  14. 14.
    Thomas RC (1970) New design for sodium sensitive glass microelectrode. J Physiol 210: 82Google Scholar
  15. 15.
    Thomas RC (1974) Intracellular pH of snail neurones measured with a new pH-sensitive glass microelectrode. J Physiol 238: 159PubMedGoogle Scholar
  16. 16.
    Thomas RC (1977) The role of bicarbonate, chloride and sodium ions in the regulation of intracellular pH in snail neurones. J Physiol 273: 317PubMedGoogle Scholar
  17. 17.
    Thomas RC (1978) Ion-sensitive intracellular microelectrodes: How to make and use them. Academic Press, London-New York-San FranciscoGoogle Scholar
  18. 18.
    Vercesi A, Reynafarje B, Lehringer Al (1978) Stoichiometry of H+ ejection and Ca2+ uptake coupled to electron transport in rat heart mitochondria. J Biol Chem 253: 6379PubMedGoogle Scholar
  19. 19.
    Weingart R (1977) The actions of ouabain on intracellular coupling and conduction velocity in mammalian ventricular muscle. J Physiol 264: 341PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • D. Ellis
    • 1
  • J. W. Deitmer
    • 1
    • 2
  • D. M. Beers
    • 1
    • 3
  1. 1.Department of PhysiologyMedical SchoolEdinburghScotland
  2. 2.Abteilung BiologieRuhr-UniversitätBochum 1Germany
  3. 3.Department of PhysiologyUCLA School of MedicineLos AngelesUSA

Personalised recommendations