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Intracellular pH, Na+ and Ca2+ Activity Measurements in Mammalian Heart Muscle

  • D. Ellis
  • J. W. Deitmer
  • D. M. Beers

Abstract

Ion-selective microelectrodes are now available for many ionic species so that studies are now feasible on the effects of changes in the activity of one type of ion on the activity of other ions in the cell. From initial studies on the control of intracellular Na+ activity (aNai) in cardiac tissues we became interested in transmembrane Na+–Ca2+ exchange. From our experiments we would have predicted that under some conditions a reduction of extracellular Na+, ([Na]o), would produce large charges in extracellular Ca2+ activity (aCai). These changes in aCai would however also probably change intracellular pH (pHi) as it has been shown that pressure injection of Ca2+ into snail neurones results in intracellular acidification (10). Therefore under conditions that produce large changes in internal Ca2+ the pHi would also be expected to alter. Changes in aCai could be demonstrated by the use of Ca2+-selective microelectrodes and by measuring the tension that the muscle developed.

Keywords

Purkinje Fibre Intracellular Acidification Glass Microelectrode Snail Neurone Cardiac Purkinje Fibre 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • D. Ellis
    • 1
  • J. W. Deitmer
    • 1
    • 2
  • D. M. Beers
    • 1
    • 3
  1. 1.Department of PhysiologyMedical SchoolEdinburghScotland
  2. 2.Abteilung BiologieRuhr-UniversitätBochum 1Germany
  3. 3.Department of PhysiologyUCLA School of MedicineLos AngelesUSA

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