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Testing of Drugs for Combined Mutagenesis with Sodium Nitrite in the Host-mediated Assay

  • R. Braun
  • J. Schöneich
  • D. Ziebarth
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 4)

Abstract

Mutagenic activity of the drugs analgine and aminophenazone was tested in the intraperitoneal and intrasanguine host-mediated assay after oral application together with equimolar doses of sodium nitrite. Salmonella typhimurium strain G46 was used as genetic indicator system; mice served as host animals for the bacteria. Analgine was found to be weakly mutagenic in the dose 2 mM/kg together with nitrite, aminophenazone was a strong mutagen in combination with nitrite in the doses 2 mM/kg and 0.2 mM/kg using the intrasanguine test with 1 h incubation of bacteria in the liver. In the intraperitoneal variant with 3 h incubation time of bacteria only aminophenazone was slightly mutagenic at the highest dose tested, 2 mM/kg. The relative nitrosation rate for aminophenazone was calculated by means of regression lines for mutagenic activity of dimethylnitrosamine and was found to be in the range of 2% in both systems for a dose of 2 mM/kg precursors.

Key words

Host-mediated assay Aminophenazone Analgine Sodium nitrite Nitrosation Dimethylnitrosamine Nitroso compounds In vivo formation 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • R. Braun
    • 1
  • J. Schöneich
    • 1
  • D. Ziebarth
    • 2
  1. 1.Zentralinstitut für Genetik und Kulturpflanzenforschung der AdW der DDRGaterslebenGDR
  2. 2.Zentralinstitut für Krebsforschung der AdW der DDRBerlin-BuchGDR

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