The 2-D Crystalline Cell Wall of Sulfolobus acidocaldarius: Structure, Solubilization, and Reassembly

  • H. Michel
  • D.-Ch. Neugebauer
  • D. Oesterhelt
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


A new type of bacteria was discovered by Brock et al. (1972) during their investigation of microorganisms living at high temperatures. They assigned the new bacteria to a hitherto unknown genus which they called Sulfolobus. The species Sulfolobus acidocaldarius is well characterized by its name. It is a facultatively sulfur-oxidizing microorganism, with cells which were found to be lobed in certain growth stages, and which require a hot and acid environment. Depending on the strain the bacteria grow best at a pH between 0.9 and 3.5 and at temperatures between 70° and 90°C (Brock, 1978).


Cell Wall Sodium Dodecyl Sulfate Cell Wall Protein Guanidinium Chloride Purify Cell Wall 
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  1. 1.
    Brock TD (1978) Thermophilic microorganisms and life at high temperatures, Ch 6. Springer, Berlin Heidelberg New YorkCrossRefGoogle Scholar
  2. 2.
    Brock TD, Brock KM, Belly RT, Weiss RL (1972) Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch Microbiol 84: 54–68CrossRefGoogle Scholar
  3. 3.
    Rosa de M, Gambacorta A, Mollig G, Bu’Lock JD (1974) Convergent characters of extremely thermophilic acidophilic bacteria. Experimenta 30: 866CrossRefGoogle Scholar
  4. 4.
    Hsung IC, Haug A (1975) Membrane potential of Thermoplasma acidophila. FEBS Lett 73 (1): 47–50CrossRefGoogle Scholar
  5. 5.
    Kates M, Wasserf K, Kushner DJ (1968) Radioisotopic studies of the biosynthesis of the glycerol diether lipids of Halobacterium halobium. Can J Biochem 46: 971PubMedCrossRefGoogle Scholar
  6. 6.
    Langworthy TA (1977) Long chain diglycerol tetraethers from Thermoplasma acidophilum. Biochem Biophys Acta 487: 37–50PubMedGoogle Scholar
  7. 7.
    Rottenberg H (1975) The measurement of transmembrane electrochemical proton gradients. J Bioenerg Biomembr 7: 61–74CrossRefGoogle Scholar
  8. 8.
    Searcy DG (1978) Thermoplasma acidophilum: Intracellular pH and potassium concentration. Biochim Biophys Acta 451: 278–286Google Scholar
  9. 9.
    Sleytr UB (1978) Regulär arrays of macromolecules on bacteria! cell walls: Structure, chemistry, assembly, and function. Int Rev Cytol 53: 1–64PubMedCrossRefGoogle Scholar
  10. 10.
    Weiss R (1974) Subunit cell wall of Sulfolobus acidocaldarius. J Bacteriol 118: 275–284PubMedGoogle Scholar
  11. 11.
    Woese CR, Magrum LJ, Fox GE (1978) Archaebacteria. J Mol Evol 11: 245–252CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • H. Michel
    • 1
  • D.-Ch. Neugebauer
    • 2
  • D. Oesterhelt
    • 1
  1. 1.Max-Planck-Institut für BiochemieMartinsried bei MünchenGermany
  2. 2.Max-Planck-Institut für biophysikalische Chemie (Karl-Friedrich-Bonhoeffer-Institut)Göttingen-NikolausbergGermany

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