The Reconstruction of Structure from Electron Micrographs of Randomly Oriented Particles

Abstract

Image enhaneement and reconstruction methods were applied to electron microscopy in order to overcome the problem of extraeting an unambiguous answer by looking at Single images of the strueture of interest. The enhaneement of images by averaging requires first identifying the position and orientation of each image. In many cases this requirement can be achieved (see J. Frank, Kessel at al., and Zingsheim in this volume). However, in principle, the accuracy of the information about position and orientation of images be- come statistically less and less reliable as one would like to enhance smaller struetures or higher resolutions. A direct Solution to this problem is to analyze ordered arrays.