The Reconstruction of Structure from Electron Micrographs of Randomly Oriented Particles

Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Image enhaneement and reconstruction methods were applied to electron microscopy in order to overcome the problem of extraeting an unambiguous answer by looking at Single images of the strueture of interest. The enhaneement of images by averaging requires first identifying the position and orientation of each image. In many cases this requirement can be achieved (see J. Frank, Kessel at al., and Zingsheim in this volume). However, in principle, the accuracy of the information about position and orientation of images be- come statistically less and less reliable as one would like to enhance smaller struetures or higher resolutions. A direct Solution to this problem is to analyze ordered arrays.

Keywords

Glutamine Autocorrelation Macromolecule Settling Dinates 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Crowther HA, Arnos LA (1971) J Mol Biol 60: 123PubMedCrossRefGoogle Scholar
  2. 2.
    Kam Z (1980) J Theor Biol 82: 15PubMedCrossRefGoogle Scholar
  3. 3.
    Kam Z (1977) Macromolecules 10: 927CrossRefGoogle Scholar
  4. 4.
    Bartlett PA, Bauer B, Singer SJ (1978) J Am Chem Soc 100: 5085CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • Z. Kam
    • 1
  1. 1.Polymer DepartmentWeizmann Institute of ScienceRehovotIsrael

Personalised recommendations