Culture of Normal Human Blood Cells in a Diffusion Chamber System II. Lymphocyte and Plasma Cell Kinetics

  • G. Chikkappa
  • A. L. Carsten
  • A. D. Chanana
  • E. P. Cronkite
Conference paper


Normal human blood leukocytes were cultured in Millipore diffusion chambers implanted into the peritoneal cavities of irradiated mice. The evaluation of survival and proliferation kinetics of cells in lymphocytic series suggested that the lymphoid cells are formed from transition of small and/or large lymphocytes, and the lymphoblasts from the lymphoid cells. There was also evidence indicating that some of the cells in these two compartments are formed by proliferation. The evaluation of plasmacytic series suggested that the plasma cells are formed from plasmacytoid-lymphocytes by transition, and the latter from the transition of lymphocytes. In addition, a relatively small fraction of cells in these two compartments is formed by proliferation. Mature plasma cells do not and immature plasma cells do proliferate. Estimation of magnitude of plasma cells formed in the cultures at day 18 indicated that at least one plasma cell is formed for every six normal human blood lymphocytes introduced into the culture.


Plasma Cell Lymphoid Cell Irradiate Mouse Small Lymphocyte Diffusion Chamber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Broder, S., Muul, L., Waldmann, T.A.: Suppressor cells in neoplastic diseases. J. Natl. Cancer Inst. 67, 5–11 (1978)Google Scholar
  2. 2.
    Capalbo, E.E., Makinodan, T.: Doubling time of mouse spleen cells during the latent and log phase of primary antibody response. J. Immunol. 92, 234–242 (1964)PubMedGoogle Scholar
  3. 3.
    Chikkappa, G., Carsten, A.L., Chanana, A.D., Cronkite, E.P.: Culture of normal human blood cells in diffusion chambers. I. Granulocyte survival and proliferation. Exp. Hematol. 6, 28–36 (1978)PubMedGoogle Scholar
  4. 4.
    Cronkite, E.P., Boecker, W., Carsten, A.L., Chikkappa, G., Joel, D.D., Laissue, J., Ohl, S.: The use of the diffusion chamber cultures in the study of normal and leukemic cell proliferation in man. In: Hemopoiesis in culture. Robinson, W.A. (ed.), pp. 185–199. Second International Workshop, DHEW Publication No. (NIH) 74–205. Washington, D.C: U.S. Gov. Print. Off. 1974Google Scholar
  5. 5.
    Dexter, T.M., Allen, T.D., Lajtha, L.G., Schofield, R., Lord, B.I.: Stimulation of differentiation and proliferation of hemopoietic cells in vitro. J. Cell. Physiol. 82, 461–473 (1973)PubMedCrossRefGoogle Scholar
  6. 6.
    Douglas, S.D., Fudenberg, H.H.: In vitro development of plasma cells from lymphocytes following pokeweed mitogen stimulation. A fine structural study. Exp. Cell Res. 54, 211–219 (1969)CrossRefGoogle Scholar
  7. 7.
    Elves, M.E.: The lymphocytes, 2nd ed. Chicago: Year Book Medical Publishers 1972Google Scholar
  8. 8.
    Good, R.A.: Experimental allergic brain inflammation. A morphological study. J. Neuropathol. Exp. Neurol. 9, 78–92 (1950)PubMedCrossRefGoogle Scholar
  9. 9.
    Grey, H.M., Rabellino, E., Pirofsky, B.: Immunoglobulins on the surface of lymphocytes. IV. Distribution in hypogammaglobulinemia, cellular immune deficiency, and chronic lymphocytic leukemia. J. Clin. Invest. 50, 2368–2375 (1971)PubMedCrossRefGoogle Scholar
  10. 10.
    Holub, M.: Potentialities of the small lymphocyte as revealed by hornotransplantation and autotransplantation experiments in diffusion chambers. Ann. N.Y. Acad. Sci. 99, 411–486 (1962)Google Scholar
  11. 11.
    Krompecher, E.: Beiträge zur Lehre von den Plasmazellen. Beitr. Pathol. Anat. 24, 163–180 (1898)Google Scholar
  12. 12.
    Maximov, A.: Untersuchungen über Blut und Bindegewebe. Arch. Mikrosk. Anat. 97, 283–313 (1923)CrossRefGoogle Scholar
  13. 13.
    Metcalf, D, Nossel, G.J.V., Warner, N.L., Miller, J.F.A.P., Mandel, T.E., Layten, J.E., Gutman, G.A.: Growth of B-lymphocyte colonies in vitro. J. Exp. Med. 142, 1534–1549 (1975)PubMedCrossRefGoogle Scholar
  14. 14.
    Parkhouse, R.M.E., Janossy, G., Greaves, M.F.: Selective stimulation of IgM synthesis in mouse B lymphocytes by pokeweed mitogen. Nature New Biol. 234, 21–23 (1972)Google Scholar
  15. 15.
    Sarkany, I., Gell, H.: Measuring lymphocyte transormation. Lancet 1966 I, 1264Google Scholar
  16. 16.
    Sasaski, M.J., Norman, A.: Proliferation of human lymphocytes in culture. Nature 210, 913–914 (1966)CrossRefGoogle Scholar
  17. 17.
    Urso, P., Makinodan, T.: The role of cellular division and maturation in the formation of precipitating antibody. J. Immunol. 90, 897–907 (1963)PubMedGoogle Scholar
  18. 18.
    Weinberg, S.R., Stohlman, F. Jr.: Growth of mouse yolk sac cells cultured in vivo. Br. J. Hematol. 32, 543–555 (1976)CrossRefGoogle Scholar
  19. 19.
    Winkelstein, A., Craddock, C.G.: Comparative response of normal human thymus and lymph node cells of phytohemagglutinin in culture. Blood 29, 594–607 (1967)PubMedGoogle Scholar
  20. 20.
    Wintrobe, M.M., Lee, G.R., Boggs, D.R., Bithell, T.C., Athens, J.W., Forester, J.: In: Clinical hematology, 7th ed., pp. 286–351 Philadelphia: Lea & FebigerGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • G. Chikkappa
  • A. L. Carsten
  • A. D. Chanana
  • E. P. Cronkite

There are no affiliations available

Personalised recommendations