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Culture of Normal Human Blood Cells in a Diffusion Chamber System II. Lymphocyte and Plasma Cell Kinetics

  • G. Chikkappa
  • A. L. Carsten
  • A. D. Chanana
  • E. P. Cronkite
Conference paper

Abstract

Normal human blood leukocytes were cultured in Millipore diffusion chambers implanted into the peritoneal cavities of irradiated mice. The evaluation of survival and proliferation kinetics of cells in lymphocytic series suggested that the lymphoid cells are formed from transition of small and/or large lymphocytes, and the lymphoblasts from the lymphoid cells. There was also evidence indicating that some of the cells in these two compartments are formed by proliferation. The evaluation of plasmacytic series suggested that the plasma cells are formed from plasmacytoid-lymphocytes by transition, and the latter from the transition of lymphocytes. In addition, a relatively small fraction of cells in these two compartments is formed by proliferation. Mature plasma cells do not and immature plasma cells do proliferate. Estimation of magnitude of plasma cells formed in the cultures at day 18 indicated that at least one plasma cell is formed for every six normal human blood lymphocytes introduced into the culture.

Keywords

Plasma Cell Lymphoid Cell Irradiate Mouse Small Lymphocyte Diffusion Chamber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • G. Chikkappa
  • A. L. Carsten
  • A. D. Chanana
  • E. P. Cronkite

There are no affiliations available

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