Different Classes of Granulocyte Progenitor Cells Expressed in In Vivo Plasma Clot Diffusion Chamber Cultures
Preliminary data suggest that at least two classes of granulocyte progenitor cells may be distinguished in the in vivo plasma clot diffusion chamber culture system. Based on several criteria, progenitor cells giving rise to granulocyte colonies on day 4 of culture (CFU-D4) appear to be different from those giving rise to colonies on day 7 (CFU-D7). These criteria include differences in the cycling characteristics of these progenitor cells as measured with cytosine arabinoside, in their resistance to osmotic lysis, in their size as determined by velocity sedimentation, and in their morphological development. The results suggest that the CFU-D7 represents an earlier progenitor cell than the CFU-D4 in the differentiation pathway of the granulocyte which in some respects displays behavior similar to that of the CFU-S.
Key WordsGranulopoiesis diffusion chamber colony-forming cells
Unable to display preview. Download preview PDF.
- 1.Axelxad, A.A., McLeod, D.L., Shreeve, M.M., Heath, D.S.: Properties of cells that produce erythrocytic colonies in plasma clot culture. In: Hematopoiesis in culture. Robinson, W.A. (ed.), pp. 226–234. DHEW Publication No. (NIH) 74–205. Washington, D.C.: U.S. Gov. Print. Off. 1974Google Scholar
- 8.Jacobsen, N., Broxmeyer, H.E., Grossbard, E., Moore, M.A.S.: Diversity of human granulopoietic precursor cells: Separation of cells that form colonies in diffusion chambers (CFU-D) from populations of colony-forming cells in vitro (CFU-C) by velocity sedimentation. Blood 52, 221–232 (1978)PubMedGoogle Scholar
- 10.McLeod, D.L., Shreeve, M.M., Axelrad, A.A.: Improved plasma clot culture system for production of erythrocytic colonies in vitro. Quantitative assay method for CFU-E. Blood 40, 517–534 (1974)Google Scholar
- 12.Niskanen, E., Cline, M.J.: Different classes of hemopoietic stem cells can be distinguished by osmotic resistance (Abstr.). In: Proceedings of the 21st Annual Meeting of the American Society of Hematology, Boston, Mass., December, 1978. Gruner & Stratton, New York 1978Google Scholar