The Use of Diffusion Chambers in the Study of Tumour Immunobiology and Lymphokines
Diffusion chambers were employed to study the in vivo mechanism of cytotoxicity reactions in a mammary tumor mouse model system. Chambers containing normal or tumor-immune murine spleen cells were implanted into both normal and tumor-bearing recipient mice. Spleen cells from recipient animals were subsequently assayed in three different types of cytotoxic reactions: phytohemagglutinin (PHA)-induced cytotoxicity, antibody-dependent cellular cytotoxicity (ADCC), and specifically induced, nonspecifically expressed cytotoxicity (SINEC). Lymphocyte responses of either group of recipients receiving chambers containing normal spleen cells were unaffected. Additionally, tumor-bearing recipients implanted with chambers containing tumor-immune spleen cells showed no significant changes in these cell-mediated immune assays. In sharp contrast, normal mice receiving chambers with tumor-immune spleen cells became responsive in the SINEC assay while not changing their reactivity in the other two cytotoxic assays. Since in vitro evidence exists that lymphokines are at least in part responsible for the mechanism of SINEC, we propose that the use of diffusion chambers demonstrates the in vivo relevance of these factors.
KeywordsSpleen Cell Mouse Bone Marrow Diffusion Chamber Spleen Lymphocyte 51Cr Release
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- 7.Lopez, D.M., Sigel, M.M., Herbert, L.: Enhanced killer cell activities induced by progressive tumor growth. J. Reticuloendothel. Soc. 22, 431–443 (1977)Google Scholar
- 12.Miller, A.M., Colley, D.G., McGarry, M.P.: Spleen cells from Schistosoma mansoni-infected mice produce diffusible stimulator of eosinophilopoiesis in vivo. NatureGoogle Scholar
- 13.Perlmann, P., Perlmann, H.: 51Cr release from chicken erythrocytes: an assay system for measuring the cytotoxic activity of “non-specifically” activated lymphocytes in vitro. In: In vitro methods in cell-mediated immunity. Bloom, B., Glade, P.R. (eds.). New York, Academic Press 1971Google Scholar
- 16.Tyler, W.S., Stohlman, F. Jr., Keane, J., Chovaniec, M., Howard, D.: Survival of murine hemopoietic cells in an in vivo culture system. Proc. Soc. Exp. Biol. Med. 146, 316–320 (1974)Google Scholar