Advertisement

A Receptor Assay of Long-Acting Thyroid Stimulator (LATS)

  • Y. Ochi
  • T. Hachiya
  • M. Yoshimura
  • T. Miyazaki
  • Y. Kajita
Conference paper

Abstract

Several abnormal thyroid stimulators have been found in serum of patients with Graves’ disease. These are known as LATS (long-acting thyroid stimulator) [1], LATS-protector [2], TSI (thyroid-stimulating immunoglobulin) [3], HTS (human thyroid stimulator) [4], or HTACS (human thyroid adenyl cyclase stimulator) [5]. These thyroid-stimulating immunoglobulins are believed to bind to a receptor site in the thyroid gland.

Keywords

Cholera Toxin Human Thyroid Normal Human Serum Hyperthyroid Patient Thyroidal Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Adams DD (1958) The presence of an abnormal thyroid-stimulation hormone in the serum of some thyrotoxic patients. J Clin Endocrinol Metab 18: 699–712PubMedCrossRefGoogle Scholar
  2. 2.
    Adams DD, Kennedy TH (1967) Occurrence in thyrotoxicosis of a gamma globulin which protects LATS from neutralization by an extract of thyroid gland. J Clin Endocrinol Metab 27: 173–177PubMedCrossRefGoogle Scholar
  3. 3.
    Smith BR, Hall R (1974) Thyroid stimulating immunoglobulins in Graves’ disease. Lancet 11: 427–430CrossRefGoogle Scholar
  4. 4.
    Onaya T, Kotani M, Yamada T, Ochi Y (1973) New in vitro test to detect thyroid stimulator in sera from hypothyroid patients by measuring colloid droplet and cyclic AMP. J Clin Endocrinol Metab 36: 859–866PubMedCrossRefGoogle Scholar
  5. 5.
    Origiazzi J, Williams DE, Chopra IJ, Solomon DH (1976) Human thyroid adenyl cyclase-stimulating activity in immunoglobulin G of patients with Graves’ disease. J Clin Endocrinol Metab 42: 341–354CrossRefGoogle Scholar
  6. 6.
    McKenzie JM (1967) The long-acting thyroid stimulator. Its role in Graves’ disease. Recent Progr Horm Res 21: 3–46Google Scholar
  7. 7.
    Amir SM, Carraway TF Jr, Kohn LD, Winand RJ (1973) The binding of thyrotrophin to isolated bovine thyroid plasma membranes. J Biol Chem 248: 4092–4100PubMedGoogle Scholar
  8. 8.
    Manley SW, Bourke JR, Hawker RW (1974) The thyrotrophin receptor in guinea-pig thyroid homogenate: General properties. J Endocrinol 61: 419–436PubMedCrossRefGoogle Scholar
  9. 9.
    Verrier B, Fayet G, Lissitzky G (1977) Thyrotrophin binding to and adenylate cyclase activity of porcine thyroid plasma membranes. Eur J Biochem 74: 243–252PubMedCrossRefGoogle Scholar
  10. 10.
    Medhi SQ, Nussey SS (1975) A radio-ligand receptor assay for the LATS. Biochem J 145: 105–111Google Scholar
  11. 11.
    Mukhtar ED, Smith BR, Pyle GA, Hall R, Vice P (1975) Relation of thyroid-stimulating immunoglobulins to thyroid function and effects of surgery, radioiodine, and antithyroid drugs. Lancet 1: 713–715PubMedCrossRefGoogle Scholar
  12. 12.
    Schleusner H, Kotulla P, Kruck I, Kruck G, Geissler G, Adlkofer F (1975) Comparison and evaluation of the radio-ligand receptor assay and the mouse bioassay for the detection of human thyroid stimulating immunoglobulins. In: Robbins J, Braverman LW (eds) Thyroid research. Excerpta Medica, Amsterdam, pp 414–416Google Scholar
  13. 13.
    Teng CS, Smith BR, Anderson J, Hall R (1975) Comparison of thyrotrophin receptors in membranes prepared from fat and thyroid tissue. Biochem Biophys Res Commun 66: 836–841PubMedCrossRefGoogle Scholar
  14. 14.
    Nagai Y, Hozoya T (1974) Isolation of Na+, K+-ATPase-associated plasma membrane of pig thyroid cells. J Biochem (Tokyo) 75: 1091–1101Google Scholar
  15. 15.
    Ochi Y, Yoshimura M, Hachiya T, Miyazaki T (1977) Distribution of LATS activity in immunoglobulin G subclass. Acta Endocrinol (Icbh) 85: 791–798Google Scholar
  16. 16.
    Schulman S, Armenia P (1963) Studies on thyroid proteins. The components of hog thyroid tissue, and the preparation of purified thyroglobulin, by column chromatography. J Biol Chem 238: 2723–2731Google Scholar
  17. 17.
    Doniach D, Roitt IM (1963) Thyroid auto-antibody tests and their clinical applications. In: Gell P, Coombs RRA (eds) Clinical immunology. Blackwell, OxfordGoogle Scholar
  18. 18.
    Ochi Y, Hachiya T, Miyazaki T, Kajita Y, Yoshimura M (1977) Response to LATS, TSH and cholera toxin of the thyroid gland in nude mice. Endocrinol Jpn 24:277–279CrossRefGoogle Scholar
  19. 19.
    Mori T, Fisher J, Kriss JP (1970) Studies of an in vitro binding reaction between thyroid microsomes and long acting thyroid stimulator globulin ( LATS ). J Clin Endocrinol Metab 31: 119–133PubMedCrossRefGoogle Scholar
  20. 20.
    Pinchera A, Rovis L, Grasso L, Liberti P, Martino E, Fenzi GF, Baschieri L (1970) Radioimmunological studies on the long acting thyroid stimulator. Endocrinology 87: 217–225PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • Y. Ochi
    • 1
  • T. Hachiya
    • 2
  • M. Yoshimura
    • 2
  • T. Miyazaki
    • 2
  • Y. Kajita
    • 2
  1. 1.Second Department of Internal MedicineShiga Medical UniversityOtsu, Shiga 520Japan
  2. 2.Second Department of Internal MedicineKyoto Prefectural University of MedicineKyoto 602Japan

Personalised recommendations