13N-L-Amino Acids Synthesized Enzymatically for in vivo Metabolic Studies
Short-lived, positron-emitting radionuclides of nitrogen and carbon may be generated with a cyclotron and used to label biologically active compounds such as amino acids for in vivo metabolic studies. The 10-min half-life of nitrogen-13 results in relatively small radiation exposures to the patient; but it requires that the chemist develop new and rapid procedures to synthesize adequate amounts of radiolabeled compounds.
KeywordsEnzymatic Synthesis Glutamate Dehydrogenase Cyanogen Bromide Label Amino Acid Amic Acid
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- 1.Cohen MB, Spolter L, MacDonald N, et al (1973) Production of 13N-labelled amino acids by enzymatic synthesis. In: Radiopharmaceuticals and labelled compounds, vol 1. IAEA, Vienna, pp 483–490Google Scholar
- 2.Lathrop KA, Harper PV, Rich BH, et al (1973) Rapid incorporation of short-lived cyclotron-produced radionuclides into radiopharmaceuticals. In: Radiopharmaceuticals and labelled compounds, vol 1. IAEA, Vienna, pp 471–481Google Scholar
- 4.Gelbard AS, Clarke LP, McDonald JM, et al (1975) Enzymatic synthesis and organ distribution studies with 13N-labeled L-glutamine and L-glutamic acid. Radiology 116: 127–132Google Scholar
- 6.Gelbard AS, McDonald JM, Reiman RE, et al (1975) Species differences in myocardial localization of 13N-labeled amino acids. J Nucl Med 16: 529Google Scholar
- 7.Cohen MB, Spolter L, MacDonald NS, et al (1975) Sequential enzymatic synthesis and separation of 13N-L-giutamic acid and 1%-L-alanine. In: Subramanian G, Rhodes BA, Cooper JF, and Sodd VJ (eds) Radiopharmaceuticals. Society of Nuclear Medicine, New York, pp 184–188Google Scholar