Abstract
Facilitated diffusion of CO2 under steady-state conditions is based on two simultaneous processes (a) diffusion of HCO −3 and (b) an equivalent H+ flux that is brought about by a facilitated H+ diffusion. We have previously shown that, in this latter process, proteins and other buffers can function as proton carriers in such a powerful way that facilitated H+ fluxes at neutral pH values are 1000–10,000 times greater than the fluxes of free protons (Gros and Moll, 1974; Gros et al., 1976). Inside cells, where soluble proteins and other mobile buffers are present, proton fluxes should almost exclusively occur by such a facilitated transport process. Our main aim in this study was to understand the mechanisms underlying these facilitated proton fluxes, and we have used the measurement of facilitated CO2 diffusion as a tool to obtain quantitative information on proton transport in protein solutions.
Supported by the Deutsche Forschungsgemeinschaft under Gr 489/2
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References
Anderson SR, Brunori M, Weber G (1970) Fluorescence studies of aplysia and sperm whale apomyoglobins. Biochemistry 9: 4723–4729
Gros G (1976) Die erleichterte Diffusion von CO2 in Proteinlösungen. Habilitationsschrift, Universität Regensburg
Gros G (1978) Concentration dependence of the self-diffusion of human and Lumbricus terrestris hemoglobin. Biophys J 22: 453–468
Gros G, Moll W (1971) The diffusion of carbon dioxide in erythrocytes and hemoglobin solutions. Pfliigers Arch 324: 249–266
Gros G, Moll W (1974) Facilitated diffusion of CO2 across albumin solutions. J Gen Physiol 64: 356–371
Gros G, Moll W, Hoppe H, Gros H (1976) Proton transport by phosphate diffusion — a mechanism of facilitated CO2 transfer. J Gen Physiol 67: 773–790
Gros G, Lavalette D, Moll W, Amand B, Pochon F, Gros H (1980) Manuscript in preparation Keller KH, Canales ER, Yum SI (1971) Tracer and mutual diffusion coefficients of proteins. J Phys Chem 75: 379–387
Lavalette D, Amand D, Pochon F (1977) Rotational relaxation of 70S ribosomes by a depolarization method using triplet probes. Proc Nati Acad Sci USA 74: 1407–1411
Levin Ö (1963) Electron microscope observations on some 60s erythrocruorins and their split products. J Mol Biol 6: 95–101
Lindstrom TR, Koenig SH, Boussios T, Bertles JG (1976) Intermolecular interactions of oxygenated sickle hemoglobin molecules in cells and cell-free solutions. Biophys J 16: 679–689
Meldon JH, de Koning J, Stroeve P (1978) Electrical potentials induced by CO2 gradients in protein solutions and their role in CO2 transport. Bioelectrochem Bioenerg 5: 77–87
Moll W (1962) Die Carrier-Funktion des Hämoglobins beim Sauerstoff-Transport im Erythrocyten. Pflügers Arch 275: 412–419
Moll W (1966) The diffusion coefficient of haemoglobin. Respir Physiol 1: 357–365
Riveros-Moreno V, Wittenberg JB (1972) The self-diffusion coefficients of myoglobin and hemoglobin in concentrated solutions. J Biol Chem 247: 895–901
Vinogradov SN, Shlom JM, Hall BC, Muzukami H (1977) The dissociation of Lumbricus terrestris hemoglobin: a model of its subunit structure. Biochim Biophys Acta 492: 136–155
Wyman J (1966) Facilitated diffusion and the possible role of myoglobin as a transport mechanism. J Biol Chem 241: 115–121
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© 1980 Springer-Verlag Berlin Heidelberg
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Gros, G., Gros, H., Lavalette, D., Amand, B., Pochon, F. (1980). Mechanisms of Facilitated CO2 and H+ Diffusion in Protein Solutions. In: Bauer, C., Gros, G., Bartels, H. (eds) Biophysics and Physiology of Carbon Dioxide. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67572-0_4
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DOI: https://doi.org/10.1007/978-3-642-67572-0_4
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