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Carbonic Anhydrase Activity in Intact Red Cells as Measured by Means of 18O Exchange Between CO2 and Water

  • R. E. Forster
  • N. Itada
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

The enzymatic activity of carbonic anhydrase inside the red cell is of practical importance, because it is this activity which is of physiological interest and not the enzymatic activity of a dilute lysate, which is generally determined experimentally. We define this activity (A), in terms of the reaction
$$C{O_2} + {H_2}O \rightleftharpoons {H_2}C{O_3}$$
as the specific rate of hydration or dehydration inside the cell divided by the same specific rate in the extracellular fluid or plasma.

Keywords

Carbonic Anhydrase Carbonic Anhydrase Activity Bonic Anhydrase Step Ratio Cell Carbonic Anhydrase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Booth VH (1938) Carbonic anhydrase activity inside corpuscles. Enzyme-substrate acessibility factors. J Physiol (London) 93: 117–128Google Scholar
  2. Constantine HP, Craw MR, Forster RE (1965) Rate of the reaction of carbon dioxide with human red blood cells. Am J Physiol 208: 801–811Google Scholar
  3. Forster RE (1964) Rate of gas uptake by red cells. In: Fenn WO, Rahn H (eds) Handbook of physiology. Respiration, vol I. American Physiological Society, Washington, pp 827–837Google Scholar
  4. Forster RE (1969) The rate of CO2 equilibration between red cell and plasma. In: Forster RE, Edsall JT, Otis AB, Roughton FJW (eds) CO2: Chemical, biochemical, and physiological aspects. National Aeronautics and Space Administration, Washington, pp 275–284Google Scholar
  5. Funder J, Wieth JO (1966) Chloride and hydrogen ion distribution between human red cells and plasma. Acta Physiol Scand 68: 234–245CrossRefGoogle Scholar
  6. Itada N, Forster RE (1977) Carbonic anhydrase activity in intact red blood cells measured with 180 exchange. J Biol Chem 252: 3881–3890Google Scholar
  7. Itada N, Peiffer L, Forster RE (1978) Intracellular enzyme activity. In: Frontiers of biological energetics, vol I. Academic Press, London New York, pp 715–724Google Scholar
  8. Kernohan JC (1964) The activity of bovine carbonic anhydrase in imidazole buffers. Biochim Biophys Acta 81: 346–356Google Scholar
  9. Kernohan JC, Roughton FJW (1966) Thermal measurements of the enzymatic activity of carbonic anhydrase in concentrated haemoglobin solutions and in red blood cells. J Physiol (London) 186: 138–139 PGoogle Scholar
  10. Kernohan JC, Forrest WW, Roughton FJW (1963) The activity of concentrated solutions of carbonic anhydrase. Biochim Biophys Acta 67: 31–41CrossRefGoogle Scholar
  11. Maren TH, Rayburn CS, Liddell NE (1976) Inhibition by anions of human red cell carbonicanhydrase B: Physiological and biochemical implications. Science 191: 469–472Google Scholar
  12. Mills GA, Urey HC (1940) The kinetics of isotopic exchange between carbon dioxide, bicarbonateion, carbonate ion and water. J Am Chem Soc 62: 1019–1026CrossRefGoogle Scholar
  13. Roughton FJW (1943–1944) Some recent work on the chemistry of carbon dioxide transport by the blood. Harvey Lect 39: 96–142Google Scholar
  14. Silverman DN, Tu C, Wynns GC (1976) Depletion of 180 from C18O2 in erythrocyte suspensions. The permeability of the erythrocyte membrane to CO2. J Biol Chem 251: 4428–4435Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • R. E. Forster
    • 1
  • N. Itada
    • 2
  1. 1.Department of Physiology G4 School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Kokuritsu Kyoto HospitalFushimi-ku, Kyoto, 612Japan

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