Carbonic Anhydrase Activity in Intact Red Cells as Measured by Means of 18O Exchange Between CO2 and Water

  • R. E. Forster
  • N. Itada
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


The enzymatic activity of carbonic anhydrase inside the red cell is of practical importance, because it is this activity which is of physiological interest and not the enzymatic activity of a dilute lysate, which is generally determined experimentally. We define this activity (A), in terms of the reaction
$$C{O_2} + {H_2}O \rightleftharpoons {H_2}C{O_3}$$
as the specific rate of hydration or dehydration inside the cell divided by the same specific rate in the extracellular fluid or plasma.


Carbonic Anhydrase Carbonic Anhydrase Activity Bonic Anhydrase Step Ratio Cell Carbonic Anhydrase 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • R. E. Forster
    • 1
  • N. Itada
    • 2
  1. 1.Department of Physiology G4 School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Kokuritsu Kyoto HospitalFushimi-ku, Kyoto, 612Japan

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