Some Properties of Cortical Nerve Cells of Adult Rats Bulk Isolated After Brain Perfusion
Several methods for the bulk separation of nerve cells have been developed during the last 20 years. Basically, two procedures are modified in one or another step [5, 6]: First, by mechanical disaggregation of the brain tissue by chopping and then sieving; and secondly, by incubating the brain tissue between the two mechanical steps, with or without exogenous enzymes.
KeywordsSucrose Trypsin Fructose Hexose HYALURONI DASE
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