Some Properties of Cortical Nerve Cells of Adult Rats Bulk Isolated After Brain Perfusion

  • H.-H. Althaus
  • W. B. Huttner
Conference paper


Several methods for the bulk separation of nerve cells have been developed during the last 20 years. Basically, two procedures are modified in one or another step [5, 6]: First, by mechanical disaggregation of the brain tissue by chopping and then sieving; and secondly, by incubating the brain tissue between the two mechanical steps, with or without exogenous enzymes.


Nerve Cell HYALURONI DASE Brain Perfusion Intact Plasma Membrane Perfusion Method 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • H.-H. Althaus
    • 1
  • W. B. Huttner
    • 2
  1. 1.Max-Planck-Institut für experimentelle MedizinForschungsstelle NeurochemieGöttingenGermany
  2. 2.Yale University School of MedicineNew HavenUSA

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