Some Properties of Cortical Nerve Cells of Adult Rats Bulk Isolated After Brain Perfusion

  • H.-H. Althaus
  • W. B. Huttner
Conference paper

Abstract

Several methods for the bulk separation of nerve cells have been developed during the last 20 years. Basically, two procedures are modified in one or another step [5, 6]: First, by mechanical disaggregation of the brain tissue by chopping and then sieving; and secondly, by incubating the brain tissue between the two mechanical steps, with or without exogenous enzymes.

Keywords

Sucrose Trypsin Fructose Hexose HYALURONI DASE 

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References

  1. 1.
    Althaus HH, Huttner WB, Neuhoff V (1977) Neurochemical and morphological studies of bulk-isolated rat brain cells. I. A new approach to the preparation of cerebral neurones. Hoppe Seylers. Z Physiol Chem 358:1155–1159CrossRefGoogle Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • H.-H. Althaus
    • 1
  • W. B. Huttner
    • 2
  1. 1.Max-Planck-Institut für experimentelle MedizinForschungsstelle NeurochemieGöttingenGermany
  2. 2.Yale University School of MedicineNew HavenUSA

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