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Methodologic Limitations on the Measurement of Thyroxine from Small Dried Blood Discs: Comparison of Double-Antibody and Solid Phase Radioimmunoassays

  • P. G. Walfish
  • E. Gera
  • M. M. Wood
Conference paper

Abstract

Measuring T4 from the eluate of 10-mm diameter dried blood samples was initially used in screening for neonatal hypothyroidism (Dussault and Laberge 1973; see also related article p. 167ff in this volume). We have previously reported about methodologic factors and limitations involved in using such a technique, involving a dried blood disc 10–13 mm in diameter (Walfish 1975, 1976; Walfish et al. 1975). Using a smaller disc of only 1/8 in. (3.2 mm) in diameter was subsequently proposed (Larsen and Broskin 1975) and has a number of theoretical advantages, as can be seen in Table 1. We therefore developed our own double-antibody radioimmunoassay (MS) to measure T4 in a single 1/8-in. diameter disc and compared our results to a commercial solid phase radioassay (Concept 4, Micromedic System).

Keywords

Congenital Hypothyroidism Mount Sinai Hospital Capillary Blood Sample Solid Phase Assay Solid Phase Radioimmunoassay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • P. G. Walfish
  • E. Gera
  • M. M. Wood

There are no affiliations available

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