Independent Requirements for Early and Late Stages of Erythropoiesis
Relatively primitive (BFU-E) and relatively mature (CFU-E) red cell precursors can be distinguished by their differing clonogenic properties in culture [1,2]. Both are dependent on erythropoietin and also on serum for colony formation. For CFU-E, the erythropoietin requirement is in keeping with a similar dependence in the whole animal [1, 3]. Their serum requirement reflects only non-specific needs, namely albumin, transferrin, unsaturated fatty acid and cholesterol [4, 5]. The requirements of BFU-E are more puzzling. These cells show no dependence on erythropoietin in the whole animal [1, 3], while their proliferative activity is subject to other control mechanisms not involving erythropoietin . In addition, they require serum for growth in culture even in the presence of albumin, transferrin and lipids. On the basis of these observations, it was suggested that the action of erythropoietin might be confined to later stages of erythroid maturation subsequent to BFU-E [3, 6, 7].
KeywordsConditioned Medium Aplastic Anemia Mouse Bone Marrow Erythroid Precursor Mouse Spleen Cell
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- 1.Axelrad, A. A., McLeod, D. L., Shreeve, M. M., Heath, D. S.: Properties of cells that produce erythrocytic colonies in vitro. In: Hemopoiesis in Culture (ed. W. A. Robinson), p. 26. U. S. Government Printing Office, Washington 1974Google Scholar
- 2.Iscove, N. N., Sieber, F., Winterhalter, K. H.: Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin-A. J. Cell. Physiol. 83, 309–320 (1974)PubMedCrossRefGoogle Scholar
- 5.Iscove, N. N., Guilbert, L. J., Weyman, C.: Complete replacement of serum in primary cultures of erythropoietin-independent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterol. Cell and Tissue Kinetics, in pressGoogle Scholar
- 6.Iscove, N. N.: Regulation of proliferation and maturation at early and late stages of erythroid differentiation. In: Cell Differentiation and Neoplasia, p. 195–209. (ed. Saunders, G. F.). New York: Raven Press 1978Google Scholar
- 7.Iscove, N. N., Guilbert, L. J.: Erythropoietin-independence of early erythropoiesis and a two-regulator model of proliferative control in the hemopoietic system. In: In vitro aspects of erythropoiesis. (ed. Murphy, M. J.), p. 3–20. New York: Springer 1978Google Scholar
- 8.Axelrad, A. A., McLeod, D. L., Suzuki, S., Shreeve, M. M.: Regulation of the population size of erythropoietic progenitor cells. In: Differentiation of normal and neoplastic hematopoietic cells. (eds. Clarkson, B., Marks, A., Till, J. E.), p. 155–163. Cold Spring Harbor Lab. 1978Google Scholar
- 9.Iscove, N. N.: Erythropoietin-independent stimulation of early erythropoiesis in adult marrow cultures by conditioned media from lectin-stimulated mouse spleen cells. In: Hematopoietic cell differentiation. (eds. Golde, D. W., Cline, M. J., Metcalf, D., Fox, C. F.), p. 37–52. New York: Acad. Press 1978Google Scholar