Independent Requirements for Early and Late Stages of Erythropoiesis

  • N. N. Iscove
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 24)


Relatively primitive (BFU-E) and relatively mature (CFU-E) red cell precursors can be distinguished by their differing clonogenic properties in culture [1,2]. Both are dependent on erythropoietin and also on serum for colony formation. For CFU-E, the erythropoietin requirement is in keeping with a similar dependence in the whole animal [1, 3]. Their serum requirement reflects only non-specific needs, namely albumin, transferrin, unsaturated fatty acid and cholesterol [4, 5]. The requirements of BFU-E are more puzzling. These cells show no dependence on erythropoietin in the whole animal [1, 3], while their proliferative activity is subject to other control mechanisms not involving erythropoietin [3]. In addition, they require serum for growth in culture even in the presence of albumin, transferrin and lipids. On the basis of these observations, it was suggested that the action of erythropoietin might be confined to later stages of erythroid maturation subsequent to BFU-E [3, 6, 7].


Conditioned Medium Aplastic Anemia Mouse Bone Marrow Erythroid Precursor Mouse Spleen Cell 
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© Springer-Verlag Berlin Heidelberg 1979

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  • N. N. Iscove

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