Abstract
Studies were undertaken to characterize specific pathophysiological defects in the preleukemic disorders in light of our present understanding of the humoral and cellular regulation of normal granulopoiesis. Studies were conducted in blast crisis of chronic myelogenous leukemia (CML), Fanconi’s anemia, aplastic anemia, and preleukemia (as characterized by a mildly megaloblastic, packed marrow and peripheral pancytopenia). Peripheral blood and bone marrow cells from such patients were studied for their ability to produce and respond to humoral regulatory factors using the semi-solid colony forming assay system in agar-gel. These studies indicate that the defect in blast crisis of CML may involve varying mechanisms in different patients, the end result of which is always a rapidly accumulating population of immature cells. In Franconi’s anemia a specific defect in the production of granulocyte colony stimulating factor has been found. In aplastic anemia our studies indicate that there is a loss of bone marrow stem cells with retention of normal production of humoral regulators. Preleukemia, on the basis of the studies reported here, appears to involve defects in both colony stimulating activity production and stem cell responsiveness to colony stimulating factors.
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References
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© 1979 Springer-Verlag Berlin Heidelberg
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Robinson, W.A., Bolin, R., Rhodes, C.A. (1979). In Vitro Disorders of Granulopoiesis in Preleukemia. In: Schmalzl, F., Hellriegel, KP. (eds) Preleukemia. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67470-9_19
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DOI: https://doi.org/10.1007/978-3-642-67470-9_19
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