Phenotypic Expression in T-Lymphocyte x “Fibroblast” Cell Hybrids
T lymphocyte x fibroblast hybrids were constructed for use in genetic and regulatory studies of T cell characteristics including immune function. The demonstration that hybrid cell lines generated by the fusion of T cells with a dedifferentiated fibroblast line continue to express T lymphocyte facultative markers is critical to the efficacy of this approach. In this communication we present the results of phenotypic analysis of permanent cell lines derived from Sendai virus mediated fusion of mixed leukocyte culture primed murine T lymphocytes or unstimulated thymocytes and line 613 fibroblasts. The latter is an L cell subline that lacks hypoxanthine-guanine phosphoribosyl transferase (HPRT), thymidine kinase (TK) and adenine phosphoribosyl transferase (APRT). Independent hybrids were examined for the expression of T cell and thymocyte facultative markers as well as the constitutive H-2 antigen specificities of the parental cells.
KeywordsTerminal Deoxynucleotidyl Transferase Somatic Cell Hybrid Hybrid Line Permanent Cell Line Adenine Phosphoribosyl Transferase
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