Advertisement

Phenotypic Expression in T-Lymphocyte x “Fibroblast” Cell Hybrids

  • N. Saravia
  • R. I. DeMars
  • F. J. Bollum
  • F. H. Bach
Conference paper

Abstract

T lymphocyte x fibroblast hybrids were constructed for use in genetic and regulatory studies of T cell characteristics including immune function. The demonstration that hybrid cell lines generated by the fusion of T cells with a dedifferentiated fibroblast line continue to express T lymphocyte facultative markers is critical to the efficacy of this approach. In this communication we present the results of phenotypic analysis of permanent cell lines derived from Sendai virus mediated fusion of mixed leukocyte culture primed murine T lymphocytes or unstimulated thymocytes and line 613 fibroblasts. The latter is an L cell subline that lacks hypoxanthine-guanine phosphoribosyl transferase (HPRT), thymidine kinase (TK) and adenine phosphoribosyl transferase (APRT). Independent hybrids were examined for the expression of T cell and thymocyte facultative markers as well as the constitutive H-2 antigen specificities of the parental cells.

Keywords

Terminal Deoxynucleotidyl Transferase Somatic Cell Hybrid Hybrid Line Permanent Cell Line Adenine Phosphoribosyl Transferase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Saravia, N., DeMars, R., Dunlap, B., Bach, F.H.: A T-cell marker in mouse fibroblast x T lymphocyte somatic cell hybrids. Scand. J. Immunol. 6, 1333–1337 (1977)PubMedCrossRefGoogle Scholar
  2. 2.
    Peck, A.B., Bach, F.H.: Mouse cell-mediated lympholysis assay in serum free and mouse serum supplemented media: culture conditions and genetic factors. Scand. J. Immunol. 4, 53–62 (1975)PubMedCrossRefGoogle Scholar
  3. 3.
    Leclerc, J.C., Levy, J.P., Varet, B., Oppenheim, S., Senik, A.: Antigenic analysis of L strain cells: A new murine leukemiaassociated antigen, “L”. Cancer Res. 30, 2073–2079 (1970)PubMedGoogle Scholar
  4. 4.
    Fenyo, E.A., Grundner, G., Klein, E.: Virus-associated surface antigens on L cells and Moloney lymphoma cells. J. Natl. Cancer Inst. 52, 743–751 (1974)PubMedGoogle Scholar
  5. 5.
    Martin, W.J.: Immune surveillance directed against derepressed cellular and viral alloantigens. Cell Immunol. 15, 1–10 (1975)PubMedCrossRefGoogle Scholar
  6. 6.
    Pellegrino, M.A., Ferrone, S., Brautbar, C., Hayflick, L.: Changes in HL-A antigen profiles on SV40 transformed human fibroblasts. Exp. Cell Res. 97, 340–396 (1976)PubMedCrossRefGoogle Scholar
  7. 7.
    Garrido, F., Schirrmacher, V., Festenstein, H.: H-2 like specificities of foreign haplotypes appearing on a mouse sarcoma after vaccinia virus infection. Nature 259, 228–230 (1976)PubMedCrossRefGoogle Scholar
  8. 8.
    Trowbridge, I.S., Ralph, P., Bevan, M.J.: Differences in the surface proteins of mouse B and T cells. Proc. Natl. Acad. Sci. USA 72, 157–161 (1975)PubMedCrossRefGoogle Scholar
  9. 9.
    Gahmberg, C.G., Hayry, P., Andersson, L.C.: Characterization of surface glycoproteins of mouse lymphoid cells. J. Cell. Biol. 68, 642–660 (1976)PubMedCrossRefGoogle Scholar
  10. 10.
    Dunlap, B., Bach, F.H., Bach, M.L.: Cell surface changes in alloantigen activated T lymphocytes. Nature 271, 253–255 (1978)Google Scholar
  11. 11.
    Gregoire, K.E., Goldschneider, I., Barton, R.W., Bollum, F.J.: Intracellular distribution of terminal deoxynucleotidyl transferase in rat bone marrow and thymus. Proc. Natl. Acad. Sci. USA 74, 3993–3996 (1977)PubMedCrossRefGoogle Scholar
  12. 12.
    Gregoire, K.E., Goldschneider, I., Barton, R.W., Bollum, F.J.: Federation Proceedings 36, 1301 (1977)Google Scholar
  13. 13.
    Bollum, F.J.: Antibody to terminal deoxynucleotidyl transferase. Proc. Natl. Acad. Sci. USA 72, 4119–4122 (1975)PubMedCrossRefGoogle Scholar
  14. 14.
    Barton, R., Goldschneider, I., Bollum, F.J.: The distribution of terminal deoxynucleotidyl transferase (TdT) among subsets of thymocytes in the rat. J. Immunol. 116, 462–468 (1976)PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • N. Saravia
  • R. I. DeMars
  • F. J. Bollum
  • F. H. Bach

There are no affiliations available

Personalised recommendations