Characterization and Functional Analysis of T Cell Hybrids
Cell hybridization of selected T lymphocyte populations with T tumor cells could provide a useful tool for the establishment of permanent tissue culture lines which display the immunological characteristics of the T lymphocytes such as antigen reactivity and surface characteristics. The rationale for employing T lymphomas for production of T hybrids is the possibility that expression of particular T lymphocyte functions may only be allowed by hybridization with tumor cells the differentiation stage of which is similar to the one of the T lymphocyte. This notion is supported by the observation that hybridization of murine T cells with the myeloma P3-X63-Ag8 results in the formation of hybrids which do not express T cell specific markers such as Thy-1 while the immunoglobulin chains of the myeloma are still secreted (Grützmann and Hämmerling, unpublished). Therefore, the AKR derived thymoma BW5147 (hypoxanthine guanine phosphoribosyl transferase negative, a gift of Dr. R. Hyman, La Jolla) was used.
KeywordsSpleen Cell Hybrid Cell Antigen Binding Sendai Virus Cell Specific Marker
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