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Rapid Binding Test for Detection of Alloantibodies to Lymphocyte Surface Antigens

  • R. M. E. Parkhouse
  • G. Guarnotta
Conference paper

Abstract

A major problem in the recently developed hybridoma technology (1) is screening very large numbers of culture supernatants for antibodies to cell surfaces. Whilst the radioactive anti-immunoglobulin (Ig) binding method has the advantage of detecting all Ig isotopes, it is nonetheless more time consuming than cytotoxic procedures. In addition it is inconvenient for detecting alloantibodies to B lymphocytes since of necessity the radioactive anti-Ig probe will bind to pre-existing B cell sIg. The modification to pre-existing procedures reported here were designed to meet these problems.

References

  1. 1.
    Köhler, G., Milstein, C.: Continuous cultures of fused cells secreting antibody of defined specificity. Nature. 256, 495–497 (1975).PubMedCrossRefGoogle Scholar
  2. 2.
    Laskey, R. A., Mills, A. D.: Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitised film. FEBS Lett. 82, 314–316 (1977).PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • R. M. E. Parkhouse
  • G. Guarnotta

There are no affiliations available

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