Rapid Binding Test for Detection of Alloantibodies to Lymphocyte Surface Antigens
A major problem in the recently developed hybridoma technology (1) is screening very large numbers of culture supernatants for antibodies to cell surfaces. Whilst the radioactive anti-immunoglobulin (Ig) binding method has the advantage of detecting all Ig isotopes, it is nonetheless more time consuming than cytotoxic procedures. In addition it is inconvenient for detecting alloantibodies to B lymphocytes since of necessity the radioactive anti-Ig probe will bind to pre-existing B cell sIg. The modification to pre-existing procedures reported here were designed to meet these problems.